Drg11-responsive (dragon) gene and uses thereof

ABSTRACT

This invention features methods and compositions useful for treating and diseases caused by a dysregulation of the BMP/GDF branch of the TGF-β signaling pathway. Also disclosed are methods for identifying compounds useful for such therapy.

CROSS REFERENCE TO RELATED APPLICATIONS

This application is a continuation of U.S. patent application Ser. No.12/489,212, filed Jun. 22, 2009, which is a continuation of U.S. patentapplication Ser. No. 11/195,205, filed on Aug. 2, 2005, which claimspriority under 35 U.S.C. §119 to of U.S. Provisional Application Ser.No. 60/598,380, filed Aug. 2, 2004, the disclosure of which isincorporated herein by reference in its entirety.

STATEMENT AS TO FEDERALLY SPONSORED RESEARCH

The present research was supported by grants from the NationalInstitutes of Health (Nos. HD039777, DK055838, HD038533, NS038253,GM075267, DK071837, and DK069533). The U.S. Government may thereforehave certain rights to this invention.

FIELD OF THE INVENTION

This invention relates to the treatment and diagnosis of diseases thatare modulated by a TGF-β-dependent signaling pathway.

BACKGROUND OF THE INVENTION

Developmentally regulated transcription factors drive developmental geneprograms that result in embryo formation and the birth, proliferation,growth, migration, and differentiation of the cells that eventually makeup the different tissues of the body. This involves the expression andrepression of many genes including those whose protein products act asregulators of this process as signal molecules. Some of these signalmolecules may be re-expressed in the adult after injury, or the failureof such re-expression may relate to the failure of replacement cells tosurvive, grow, or regenerate after injury. Some of the signal moleculesmay act in pathological situations to either promote or suppressabnormal growth or function. These signal molecules, acting on specifictransmembrane receptors, may serve as cell fate determinants, survivalfactors, growth factors, guidance cues, or differentiation factors, andmany may have potential therapeutic roles as biological agents beyondtheir specific involvement in development. Such factors can havebiological activity both in vivo and for maintaining cultured cells invitro, or for converting pluripotent stem cells into specific neuronalor non-neuronal subtypes. Similarly, mimicking the action of thesesignal molecules by activating their membrane bound receptors or theintracellular signal transduction pathways coupled to their receptors,may also have therapeutic potential.

The transforming growth factor beta (TGF-β) superfamily ligands arecentral to many signal transduction pathways that control the growth anddifferentiation of mammalian cells. These ligands and pathways have beenimplicated in the control of a variety of cellular processes rangingfrom early vertebrate development to carcinogenesis where specific TGF-βligands are involved in cell specification, differentiation,proliferation, patterning, and migration.

The TGF-β signaling pathways may be subdivided along two majorbranches—the TGF-β/Activin/Nodal pathways and the BMP/GDF pathways. TheTGF-β/activin/nodal subfamily of ligands contribute to the specificationof endoderm and mesoderm in pregastrula embryos and at gastrula stages,to dorsal mesoderm formation and anterior-posterior patterning. Later,they influence the body axis and dorsal-ventral patterning of thenervous system. Bone morphogenetic proteins (BMPs), the second majorsubfamily of TGF-β ligands contribute to the ventralization of germlayers in the early embryo, suppressing the default neural cell fate ofthe ectoderm. Neural induction follows formation of the organizer in thedorsal mesoderm which generates inhibitory signals that interrupt BMPsignaling in the ectoderm leading to a separation of neural fromepidermal territories. BMPs also participate later in development in theformation and patterning of the neural crest, heart, blood, kidney,limb, muscle, and skeletal system.

SUMMARY OF THE INVENTION

We have discovered that DRAGON (DRG11-Responsive Axonal Guidance andOutgrowth of Neurite) proteins, including RGMa and RGMb, theprototypical members of a novel gene family (WO 03/089608), function asco-receptors for both BMP ligands and BMP ligand receptors andfacilitate enhanced BMP signaling. Homologous DRAGON proteins have beenidentified in each of the mouse, zebrafish, and human (WO 03/089608). Apartial sequence of an ortholog has also been identified in C. elegans.

Accordingly, the invention features a method for identifying a compoundthat modulates a TGF-β signaling pathway, preferably a BMP/GDF pathway,by (a) providing a sample containing DRAGON protein and a TGF-βsignaling pathway member, (b) contacting the sample with a candidatecompound, and (c) assessing the binding of the DRAGON protein to theTGF-β signaling pathway member in the sample in the presence of thecandidate compound relative to binding in the absence of the candidatecompound, wherein a compound that modulates binding of the DRAGONprotein to the TGF-β signaling pathway member is identified as acompound that modulates a TGF-β signaling pathway. Preferably, the TGF-βsignaling pathway member is a BMP (e.g., BMP-2 and BMP-4), GDF5, a typeI BMP receptor (e.g., ALK2, ALK3, and ALK6), or a type II BMP receptor(e.g., BMPRII, ActRIIA, and ActRIIB). More preferably, the TGF-βsignaling pathway member is labeled with a radioisotope (e.g.,[¹²⁵I-BMP-2 and [¹²⁵I]-BMP-4) in order to facilitate the assessing step(c). In another embodiment, the assessing step (c) uses an antibodyspecific for DRAGON (i.e., an anti-DRAGON antibody) or an antibodyspecific for the TGF-β signaling pathway member.

The invention also features a second method for identifying a compoundthat modulates a TGF-β signaling pathway, preferably a BMP/GDF pathway,by (a) providing a cell that expresses, naturally or recombinantly, atype I BMP receptor, a type II BMP receptor, and an intracellular TGF-βsignaling pathway member, (b) contacting the cell with DRAGON and acandidate compound, and (c) assessing the level of activation of theTGF-β signaling pathway by assessing the activation of the intracellularTGF-β signaling pathway member relative to the level of activation inthe absence of the candidate compound, wherein a compound that modulatesthe activation of the intracellular TGF-β signaling pathway member isidentified as a compound that modulates a TGF-β signaling pathway.Preferably, the type I BMP receptor is ALK2, ALK3, or ALK6, and the typeII BMP receptor is BMPRII, ActRIIA, or ActRIIB Preferably, theintracellular TGF-β signaling pathway member is an R-Smad (e.g., Smad1,Smad5, and Smad8). Preferably, step (c) assesses the phosphorylationstate of the intracellular TGF-β signaling pathway member or the bindingof the intracellular TGF-β signaling pathway member to anotherintracellular protein such as a Co-Smad (e.g., Smad4 and Smad4β) as anindicator of TGF-β signaling pathway activation. In another embodiment,the cell is further contacted, in step (b), with a TGF-β ligand such as,for example, BMP-2, BMP-4, BMP-7, or GDF-5. Contacting the cell withDRAGON in step (b) may be performed either through the addition ofexogenous DRAGON or by transfecting the cell with a nucleic acid capableof expressing DRAGON.

The invention yet further features a third method for identifying acompound that modulates a TGF-β signaling pathway, preferably a BMP/GDFpathway, by (a) providing a cell that expresses a reporter geneconstruct operably linked to a TGF-β ligand-dependent promoter, (b)contacting the cell with DRAGON protein and a candidate compound, and(c) assessing the level of expression of the reporter gene relative tothe level of expression of the reporter gene in the absence of thecandidate compound, wherein a candidate compound that modulates thelevel of expression of the reporter gene is identified as a compoundthat modulates a TGF-β signaling pathway. In preferred embodiments, thereporter construct is BMP-dependent. In other preferred embodiments, thereporter construct is the BRE-Luc reporter. In other embodiments, thecell is further contacted, in step (b), with a TGF-β ligand such as, forexample, BMP-2, BMP-4, BMP-7, or GDF-5. In other embodiments, the cellfurther expresses recombinant DRAGON. Optionally, the cell alsoexpresses, naturally or recombinantly, one or more BMP type I receptors(e.g., ALK2, ALK3, and ALK6) and/or one or more the type II BMP receptor(e.g., BMPRII; ActRIIA, and ActRIIB).

In another aspect, the invention features a method for identifying acompound that modulates cellular adhesion by (a) providing a samplecontaining a DRAGON protein and an adhesion-modulating protein, (b)contacting the sample with a candidate compound, and (c) assessing thebinding of the DRAGON protein to the adhesion-modulating protein in thesample in the presence of the candidate compound relative to binding inthe absence of the candidate compound, wherein a compound that modulatesbinding of the DRAGON protein to the an adhesion-modulating protein isidentified as a compound that modulates cellular adhesion. In preferredembodiments, the adhesion-modulating protein is a cadherin (e.g.,E-cadherin), a second DRAGON protein (a homophilic interaction), or aDRAGON-like protein (e.g., DL-N or DL-M). In other embodiments, theDRAGON protein and the adhesion-modulating protein are bound tomicrospheres or are present on the plasma membrane of a cell.

Compounds identified by any of the screening methods described hereinfind use in diagnosis and therapy of any DRAGON-related conditiondescribed herein, or as lead compounds for optimizing therapeutics forthose conditions. Compounds identified by the present screens also findreproductive-related uses, such as treating infertility by increasingDRAGON activity or providing contraceptives by decreasing DRAGONactivity.

In another aspect, the invention features a method for diagnosing cancerin a patient by assessing the amount of DRAGON is a biological sample.Suitable biological samples include, for example, blood samples, tissuebiopsies, pleural fluid, and cerebrospinal fluid. This method isparticularly useful for diagnosing colon cancer, breast cancer,testicular cancer, ovarian cancer, and neuronal and non-neuronal cancersof the nervous system (e.g., glioma, schwanoma, and neuroblastoma). Theamount of DRAGON in a biological sample may be assessed by any techniqueknown in the art including, for example, DRAGON-specific antibody-basedassays (e.g., ELISA, Western blotting, and immunohistochemistry) andDRAGON-specific primer/probe-based molecular biological techniques usingDRAGON-specific polynucleotides (e.g., in situ hybridization or PCRfollowed by Northern blotting). In preferred embodiments, a DRAGONprotein or DRAGON RNA is visualized for its intracellular localization.

In another aspect, the invention features a method for assessing thelikelihood of a patient to develop cancer by identifying a DRAGONmutation that correlates with a propensity to develop cancer. Suchmutations may cause a change (i.e., increase or decrease) in DRAGONbiological activity. Mutations in the GPI anchoring domain that aresufficient to disrupt the membrane anchoring of DRAGON are examples ofmutations that reduce DRAGON biological activity and increase thelikelihood of cancer. Other mutations may be located in the BMP ligandbinding domain, the BMP receptor binding domain, or the cadherin bindingdomain. Such mutations may be polymorphic changes.

In yet another aspect, chimeric proteins consisting of a DRAGON proteinor fragment or DRAGON-specific ligands, or an anti-DRAGON antibody, maybe covalently linked to a cytotoxic moiety may be used to treat cancer.Useful cytotoxic moieties include, for example, saporin, Pseudomonasexotoxin, IL-12, TNF-α, and radioisotopes. Another useful moiety isboron which may be used to kill target cells using neutron capturetherapy. The chimeric protein may be administered to the cancer cellfrom an exogenous source, or the chimeric protein may be expressed bythe cancer cell following, for example, administration of atherapeutically effective amount of a nucleic acid encoding the chimericprotein, operably linked to a promoter that, when expressed in thecancer cells, is capable of expressing the chimeric protein.

The invention also features a method for treating a DRAGON-relatedcondition in a patient by increasing the DRAGON activity in the patient.DRAGON activity may be increased by administering a compound capable ofincreasing DRAGON binding to a TGF-β signaling pathway member such as aTGF-β ligand (e.g., BMP-2, BMP-4, and GDF-5), or a compound capable ofincreasing DRAGON binding to a), a BMP type I receptor (e.g., ALK2, ALK4and ALK6), or a BMP type II receptor (e.g., BMPRII, ActRIIA, andActRIIB). Preferably, the compound has been previously identified by oneof the foregoing screening methods. In preferred embodiments, theincreased DRAGON activity causes increased signaling in the BMP/GDFpathway. In other embodiments, the method further comprisesadministering a biologically active BMP-2, BMP-4, or GDF-5 polypeptide.In another embodiment, the patient is administered a therapeuticallyeffective amount of a nucleic acid encoding a DRAGON protein, operablylinked to a promoter that, when expressed in the target cells, iscapable of expressing the DRAGON protein. DRAGON-related conditionsamenable to treatment using these methods include, for example, cancerscharacterized as having a defect in the BMP/GDF signaling pathway.Disorders of bone, cartilage, and joints may also be treated byincreasing DRAGON activity. Such disorder include, for example, bonefractures, damage to the articular cartilage, arthritis, chondroplasia,and synostosis. Pulmonary hypertensions, particularly familiar primarypulmonary hypertension, kidney disorders (e.g., ischemic kidneydisorders and renal fibrosis), male infertility, female infertility, anddisorders of the thymus also can be treated by these methods.

The invention also features a method for treating a DRAGON-relatedcondition in a patient by decreasing the DRAGON activity in the patient.DRAGON activity may be reduced by administering a soluble DRAGON protein(i.e., a DRAGON protein having a deletion or disruption of the GPImembrane anchoring domain) in order to sequester BMP ligands or bind(occupy) a membrane-bound DRAGON, an anti-DRAGON antibody or antibodyfragment, a compound capable of inhibiting DRAGON binding to a TGF-βligand (e.g., BMP-2, BMP-4, and GDF-5), a BMP type I receptor (e.g.,ALK2, ALK4 and ALK6), or a BMP type II receptor (e.g., BMPRII, ActRIIA,and ActRIIB). Preferably, the compound has been previously identified byone of the foregoing screening methods. In another embodiment, thepatient is administered a therapeutically effective amount of aDRAGON-specific RNAi sufficient to inhibit DRAGON expression. DRAGONactivity may also be reduced by administering to the patient a nucleicacid encoding an antisense DRAGON nucleic acid or a soluble DRAGONprotein. Certain types of cancers, such as breast cancer, colon cancer,non-small cell lung carcinoma, and neuronal and non-neuronal cancers ofthe nervous system (e.g., glioma, schwanoma, and neuroblastoma) areamenable to treatment by these methods. These methods may also be usedto inhibit tumor metastasis in a patient diagnosed as having cancer. Inother embodiments, the patient is further administered an inhibitor of aTGF-β ligand, a BMP type I receptor, or BMP type II receptor. Inhibitorsof TGF-β ligands include ligand-specific antibodies and soluble BMPreceptors and fragments. Inhibitors of the various BMP receptors alsoinclude receptor-specific antibodies, mutated TGF-β ligands that retainaffinity and lack efficacy, and compounds that inhibit the kinasefunction of the receptor.

In another aspect, the invention features a method for inhibitingsignaling by a BMP by administering a soluble DRAGON protein or aDRAGON-Fc fusion protein. In preferred embodiments, the BMP is BMP-2 orBMP-4.

By “DRAGON” is meant any naturally occurring DRAGON homolog andbiologically active fragments thereof. The DRAGON protein has beenidentified in several species including the mouse, human, C. elegans,and zebrafish (SEQ ID NOs: 1-4, respectively). One particular example ofa DRAGON homolog is RGMa (see FIG. 24).

By “DRAGON nucleic acid” is meant a polynucleotide having a sequencewhich encodes a DRAGON protein, for example RGMa or RGMb. Preferably, aDRAGON nucleic acid is substantially identical or hybridizes under highstringency conditions to a DRAGON cDNA from, for example, the mouse,human, or zebrafish (SEQ ID NOs: 5-7, respectively).

By “mutation,” when referring to a DRAGON nucleic acid is meant anyalteration in the protein coding region which results in a change in theamino acid sequence encoded. Mutations include single point mutationsand polymorphisms as well as deletions of one or more nucleic acids.Mutations may also occur in the untranslated region of a DRAGON nucleicacid such that the mutation affects biological activity. For example, amutation in the promoter region of the DRAGON gene may have the effectof reducing DRAGON expression.

By “TGF-β signaling pathway member” is meant any protein involved in anyTGF-β signal transduction pathway. These pathways include, for example,the BMP/GDF pathway and the TGF-β/Activin/Nodal pathways. The pathwaymembers include the extracellular ligands (e.g., the BMPs), thetransmembrane receptors (e.g., the BMP receptors and the TGF-βreceptors), the intracellular substrates of the transmembrane receptors(e.g., the R-Smads), and the intracellular accessory proteins (e.g., theCo-Smads and SARA).

By “high stringency conditions” is meant any set of conditions that arecharacterized by high temperature and low ionic strength and allowhybridization comparable with those resulting from the use of a DNAprobe of at least 40 nucleotides in length, in a buffer containing 0.5 MNaHPO₄, pH 7.2, 7% SDS, 1 mM EDTA, and 1% BSA (Fraction V), at atemperature of 65° C., or a buffer containing 48% formamide, 4.8×SSC,0.2 M Tris-Cl, pH 7.6, 1×Denhardt's solution, 10% dextran sulfate, and0.1% SDS, at a temperature of 42° C. Other conditions for highstringency hybridization, such as for PCR, Northern, Southern, or insitu hybridization, DNA sequencing, etc., are well-known by thoseskilled in the art of molecular biology. See, e.g., Ausubel et al.,Current Protocols in Molecular Biology, John Wiley & Sons, New York,N.Y., 2000, hereby incorporated by reference.

By “DRAGON antisense nucleic acid” is meant a nucleic acid complementaryto a DRAGON coding, regulatory, or promoter sequence. Preferably, theantisense nucleic acid decreases DRAGON expression (e.g., transcriptionand/or translation) by at least 5%, 10%, 25%, 50%, 75%, 90%, 95%, oreven 99%. Preferably, the DRAGON antisense nucleic acid comprises fromabout 8 to 30 nucleotides. A DRAGON antisense nucleic acid may alsocontain at least 40, 60, 85, 120, or more consecutive nucleotides thatare complementary to a DRAGON mRNA or DNA, and may be as long as afull-length DRAGON gene or mRNA. The antisense nucleic acid may containa modified backbone, for example, phosphorothioate, phosphorodithioate,or other modified backbones known in the art, or may contain non-naturalinternucleoside linkages.

A DRAGON antisense nucleic acid may also be encoded by a vector wherethe vector is capable of directing expression of the antisense nucleicacid. This vector may be inserted into a cell using methods known tothose skilled in the art. For example, a full length DRAGON nucleic acidsequence, or portions thereof, can be cloned into a retroviral vectorand driven from its endogenous promoter or from the retroviral longterminal repeat or from a promoter specific for the target cell type ofinterest. Other viral vectors which can be used include adenovirus,adeno-associated virus, vaccinia virus, bovine papilloma virus, or aherpes virus, such as Epstein-Ban Virus.

By “vector” is meant a DNA molecule, usually derived from a plasmid orbacteriophage, into which fragments of DNA may be inserted or cloned. Avector will contain one or more unique restriction sites, and may becapable of autonomous replication in a defined host or vehicle organismsuch that the cloned sequence is reproducible. A vector contains apromoter operably linked to a gene or coding region such that, upontransfection into a recipient cell, an RNA is expressed.

By “substantially pure” is meant a nucleic acid, polypeptide, or othermolecule that has been separated from the components that naturallyaccompany it. Typically, the polypeptide is substantially pure when itis at least 60%, 70%, 80%, 90% 95%, or even 99%, by weight, free fromthe proteins and naturally-occurring organic molecules with which it isnaturally associated. For example, a substantially pure polypeptide maybe obtained by extraction from a natural source, by expression of arecombinant nucleic acid in a cell that does not normally express thatprotein, or by chemical synthesis.

By a “promoter” is meant a nucleic acid sequence sufficient to directtranscription of a gene. Also included in the invention are thosepromoter elements which are sufficient to render promoter-dependent geneexpression controllable for cell type-specific, tissue-specific orinducible by external signals or agents (e.g. enhancers or repressors);such elements may be located in the 5′ or 3′ regions of the native gene,or within an intron.

By “operably linked” is meant that a nucleic acid molecule and one ormore regulatory sequences (e.g., a promoter) are connected in such a wayas to permit expression and/or secretion of the product (e.g., aprotein) of the nucleic acid molecule when the appropriate molecules(e.g., transcriptional activator proteins) are bound to the regulatorysequences.

By “antibody that selectively binds” is meant an antibody capable of ahigh affinity interaction with a specific target molecule, having adissociation constant of <1 μM, <100 nM, <10 nM, <1 nM, or even <100 pM.Preferably, the antibody has at least 10-fold, 100-fold, 1,000-fold, oreven 10,000-fold lower affinity for other, non-target molecules. A“DRAGON-specific antibody” is, therefore, an antibody that selectivelybinds to a DRAGON protein.

By a “DRAGON-related condition” is meant any disease or disorder whichis associated with the dysfunction or altered (increased or decreased)activity or expression of DRAGON. Alternatively, DRAGON-relatedconditions can also refer to any disease or disorder which, although notassociated with DRAGON dysfunction, is amenable to treatment bymodulating (increasing or decreasing) the activity or expression of aDRAGON protein or nucleic acid or by mimicking their actions. Typically,these DRAGON-related conditions are associated with a dysfunction insignal transduction in the BMP/GDF branch of the TGF-β signalingpathway. The dysfunction may be an inappropriate activation, inmagnitude or duration, of the signaling pathway, requiring a reductionof DRAGON biological activity. Alternatively, the signaling pathway maybe hypoactive and successful therapy requires increasing the level ofpathway activation.

By a “therapeutically effective amount” is meant a quantity of compound(e.g., a DRAGON protein) delivered with sufficient frequency to providea medical benefit to the patient. Thus, a therapeutically effectiveamount of a DRAGON protein is an amount sufficient to treat orameliorate a DRAGON-related condition or symptoms.

By “treating” is meant administering a pharmaceutical composition forthe purpose of improving the condition of a patient by reducing,alleviating, or reversing at least one adverse effect or symptom.

BRIEF DESCRIPTION OF DRAWINGS

FIG. 1 is a graph illustrating the result of a computationalstructure-function analysis of mDRAGON, demonstrating the presence of asignal sequence which results in protein secretion.

FIGS. 2A-2D provide experimental results using a novel anti-DRAGONrabbit polyclonal antibody. FIG. 2A is a Western blot analysis ofprotein extract from untransfected HEK293 cells (−), or thosetransfected (+) with DRAGON expression vector. A distinct band having amolecular weight of about 50 KDa is recognized by the anti-DRAGONantibody in transfected, but not control, cells. ERK protein level wasused as a loading control. FIG. 2B is a photomicrograph of animmunocytochemical study showing significant staining ofDRAGON-expressing HEK cells (top). Pretreatment of DRAGON-expressing HEKcells with PI-PLC causes a significant reduction of anti-DRAGON staining(bottom). Non-transfected HEK cells show no anti-DRAGON staining (notshown). FIG. 2C is a photomicrograph of a Western blot analysis ofsamples of DRAGON-expressing HEK cell culture medium, with or withoutpretreatment using PI-PLC. A band corresponding to DRAGON is detected inPI-PLC treated medium samples. FIG. 2D is a series of photomicrographsfrom an anti-DRAGON immunohistochemical study of adult spinal cord andDRG at low (top) and high (middle) magnification. As a control, theanti-DRAGON antibody was pretreated with the immunogenic DRAGON fragmentprior to immunohistochemical staining (bottom). Scale, 100 μM.

FIG. 3 is a photomicrograph showing the distribution of DRAGONexpression in the retina and optic nerve of a mouse embryo (E14.5) usingimmunohistochemistry.

FIG. 4 is a photomicrograph showing the distribution of DRAGONexpression in rat glaborous skin (base of the epidermis of the hindpaw)using immunohistochemistry. DRAGON expression is highest in the Merkelcells.

FIG. 5A is a photomicrograph of an in situ hybridization study showingthat DRAGON and DRG11 mRNAs are both expressed in the dorsal rootganglion (DRG) and the spinal cord at E12.5. FIG. 5B is a bar graphshowing the DRG11-dependent enhancer activity of the DRAGON promoterfragment. FIG. 5C shows the results of a pull-down experiment usingeither GST or GST-DBD (DBD=DRG11 DNA Binding Domain). The purifiedproteins (right panel) were incubated with the DRAGON promoter fragment,and “pulled down” using glutathione sepharose. Only GST-DBD fusionprotein pulled down the promoter fragment as assessed by agarose gelelectrophoresis. FIG. 5D is a photomicrograph of an in situhybridization study demonstrating a decrease in DRAGON mRNA expressionin the DRG and the spinal cord of DRG11−/− mouse at E14.5, compared towildtype. FIG. 5E shows the result of a Northern blot analysis of DRAGONmRNA expression in adult and embryonic E14.5 tissue. FIG. 5F shows theresult of a Northern blot analysis of DRAGON mRNA expression in wholemouse embryos during development. β-actin mRNA levels were used as aloading control.

FIG. 6 is a series of photomicrographs showing the distribution ofDRAGON mRNA in the adult rat DRG by in situ hybridization.

FIG. 7 is a series of photomicrographs showing the distribution ofDRAGON mRNA in the brain of an E18 mouse by in situ hybridization.

FIG. 8A is a series of photomicrographs showing the localization ofDRAGON during mouse embryogenesis at E2.5, visualized using ananti-DRAGON antibody and FITC fluorescence (left panel). Nuclei of thecells are visualized by CY3 fluorescence (PI; middle panel).Pre-adsorption of the DRAGON antibody with the peptide antigen was usedas a negative control (Pept.; right panel). FIG. 8B is a Northern blotshowing the developmental profile of DRAGON expression in the mouseembryo. Cyclophilin mRNA levels were measured as a loading control(lower panel). FIG. 8C are photomicrographs of whole embryo (E10.5)DRAGON immunohistochemistry. FIG. 8D is a Northern blot of embryonicXenopus DRAGON expression, using RT-PCR. ODC levels were used as aninternal control. FIG. 8E is a series of photomicrographs of wholemounted Xenopus embryos following in situ hybridization of expression ofRGMa at stages 12 (i), 17 (ii), 20 (iii), and 35 (iv). Transversesections through stained st12 and st20 embryos are also shown.(bp=balstopore; post=posterior; ant=anterior; nc=notochord; rp=roofplate; hb=hindbrain; mb=midbrain; fb=forebrain; ba=brachial arches).

FIG. 9A is a bar graph showing the effect of BMP-2 and DRAGON on BRE-Lucreporter gene activation. FIG. 9B is a bar graph showing the combinedeffects of BMP-2 and DRAGON on BRE-Luc reporter gene activation. FIG. 9Cis a bar graph demonstrating that DRAGON does not affect TGF-β- orActivin-mediated signaling.

FIG. 10A is a series of Western blots following an anti-DRAGONimmunoprecipitation, using an anti-HA antibody, that demonstrates thatDRAGON physically binds to ALK2 (A2), ALK3 (A3), and ALK6 (A6). FIG. 10Bis a series of Western blots following an anti-DRAGONimmunoprecipitation, using an anti-HA antibody, that demonstrates thatDRAGON physically binds to BMPRIIA (RII), and BMPRIIB (RIIB) FIG. 10C isa bar graph showing that DRAGON-induced signaling is mediated throughALK3 and ALK6. ALK3-DN, ALK6-DN, but not ALK1-DN expression reducesDRAGON-induced signaling. FIG. 10D is a bar graph showing thatDRAGON-induced signaling is mediated through a Smad1 dependentmechanism. BRE-Luc reporter gene activation is significantly reduced inthe presence of increasing concentrations of a dominant negative Smad1.

FIG. 11A is a bar graph demonstrating that noggin, a BMP antagonist,inhibits both BMP-2-dependent and DRAGON dependent BRE-Luc reporter geneactivation. FIG. 11B is a bar graph demonstrating that follistatin, anactivin antagonist, has no effect on BMP-2-dependent and DRAGONdependent BRE-Luc reporter gene activation. FIGS. 11C and 11D are bargraphs demonstrating the an anti-DRAGON-Fc antibody does not detectbinding between DRAGON and [¹²⁵I]-noggin.

FIG. 12A is a series of phosphoimages demonstrating that DRAGONinteracts directly with BMP2 and BMP4. COS-1 cells were transientlytransfected with DRAGON, HA-tagged ALK6 or TβRII and affinity-labeledwith [¹²⁵I]-BMP2 (left) or [₁₂₅I]-TGFβ (right) and immunoprecipitatedusing the indicated antibodies. FIG. 12B is a bar graph quantifying theamount of DRAGON-Fc binding to [¹²⁵I]-BMP2. The DRAGON Fc/[¹²⁵I]-BMP2complexes were captured on protein A coated plates and boundradioactivity was measured. FIG. 12C is a bar graph quantifying theamount of DRAGON-Fc binding to [¹²⁵I]-BMP2 in the presence of variousTGF-β family receptors. DRAGON-Fc binding to [¹²⁵I]-BMP2 is effectivelydisplaced by unlabeled BMP-2 and BMP-4, but not BMP-7, Activin A,TGF-β1, TGF-β2, or TGF-β3. FIG. 12D is a bar graph showing the relativeintensity of luciferase expression in LLC-PK1 cells transfected with theBRE-Luc reporter construct. Soluble DRAGON (DRAGON-Fc) reduces theBMP2-mediated signaling. FIG. 12E is a bar graph showing the relativeintensity of luciferase expression in LLC-PK1 cells transfected with theBRE-Luc reporter construct. Soluble DRAGON (DRAGON-Fc) reduces theBMP2-mediated signaling, but not BMP7- or TGF-β1-mediated signaling.

FIG. 13A is a bar graph demonstrating that membrane-bound DRAGON, butnot soluble DRAGON, is capable of activating BRE-Luc reporter geneexpression. FIG. 13B is a western blot using an anti-DRAGON antibody toconfirm the presence of both the membrane-bound and soluble form ofDRAGON in the previous experiment.

FIG. 14 is a schematic diagram of the interaction of DRAGON with the BMPsignaling pathway.

FIG. 15A is a series of RT-PCR Northern blots from Xenopus embryosmicroinjected with murine DRAGON mRNA. FIG. 15B is a series of RT-PCRNorthern blots from animal cap explants microinjected with DRAGON mRNA.FIG. 15C is a series photomicrographs of whole mounted Xenopus embryosfollowing injection of DRAGON mRNA into the animal pole of 1 out of 2cells at the 2-cell stage. DRAGON induces inhibits twist RNA (a neuralcrest marker) and induces ectopic N-tubulin expression (a neuralmarker).

FIG. 16 is a photomicrograph showing the expression of DRAGON in themouse knee joint.

FIG. 17 is a series of photomicrographs showing the elevated expressionof DRAGON in the ischemic kidney.

FIG. 18 is a photomicrograph showing the expression of DRAGON in themouse testis.

FIG. 19 is a photomicrograph showing the expression of DRAGON in normalbreast tissue and breast cancer tissue.

FIG. 20 is a photomicrograph showing the expression of DRAGON in thenormal colon and in colon cancer tissue.

FIGS. 21A-21C are a series of photomicrographs that demonstrate theadhesion of DRG neurons to DRAGON-expressing HEK 293 cells. P14 neonatalDRG neurons were plated on a monolayer of confluent HEK cells and DRAGONtransfected HEK cells. The culture slides were washed, fixed, andimmunostained for DRG neurons using anti-NeuN (neuronal marker). Doubleimmuno-labeling using anti-NeuN and anti-DRAGON indicates a directinteraction between DRAGON expressing HEK cells and DRG neurons (FIG.21C). FIG. 21D is a bar graph quantifying the adhesion experimentresults. A 1.9-fold increase in the number of adherent DRG neurons wasobserved when plated on DRAGON-expressing HEK 293 cells, compared tocontrol HEK 293 cells. Pretreatment of the DRAGON-expressing HEK cellswith PI-PLC significantly reduced the adherence of DRG neurons.

FIG. 22 is a schematic diagram of the role of DRAGON in cell adhesion.

FIG. 23A is a vector map of the pLenti-hU6BX expression vector designedto express double stranded shRNA to silence DRAGON gene expression. TheGFP cassette is used to check transfection and expression and theampicillin resistance gene is used to select for positive clones. FIG.23B is a Western blot on extracts from SHSY5Y neuroblastoma cellsexpressing two different shRNA vectors or a negative control. Each ofthe shRNA vectors is directed to a different region of the DRAGON cDNA.

FIG. 24 provides the amino acid (SEQ ID NOs.: 1, 2, and 4) and nucleicacid (SEQ ID NOs: 5-7) sequences of mouse, human, and zebrafish DRAGON,the amino acid sequences of C. elegans DRAGON (SEQ ID NO:3), the aminoacid (SEQ ID NOs: 8-9) and nucleic acid (SEQ ID NOs: 10-11) sequences ofhuman DL-M and DL-N, and the amino acid (SEQ ID NO:12) and nucleic acid(SEQ ID NO:13) sequences of RGMa.

FIG. 25 is a set of images of gels showing DRAGON mRNA expression inmouse tissues. RNA extracted from various mouse tissues was examined forDRAGON mRNA expression by RT-PCR. In tissues where DRAGON expression waslow, β-actin was used as a control for cDNA quality. DRAGON expressionwas strongly detected in testis (Te), ovary (Ov), pituitary (Pit),epididymis, uterus (Ut), kidney (Kid), and brain (Br). Weaker DRAGONsignals were detected in seminal vesicles (SV) and adrenal (Ad).

FIGS. 26A-26D are images showing cellular localization of DRAGON in themouse testis and epididymis during postnatal development byimmunohistochemistry and in situ hybridization. Forimmunohistochemistry, all sections were stained with DAB andcounterstained with hematoxylin. Images are shown at lower (40) orhigher (100) magnification. FIG. 26A shows immunolocalization of DRAGONin testes at d1 (D1, a), d3 (D3, b), d9 (D9, c), d21 (D21, d), and d60(D60, e and f). For negative control, sections were incubated withDRAGON antibody preincubated with competing immunizing peptide (g andh). DRAGON is highly expressed in gonocytes and spermatogonia in testesof newborn mice and spermatocytes and round spermatids in testes ofadult mice. DRAGON is not expressed in spermatocytes of d21 testes.Spermatogonia are weakly stained in d21 testes but not stained in adulttestes. FIG. 26B shows immunostaining of d21 testes with (b) and without(a) PMSG (5 IU) injection 2 d earlier. Immunostaining in spermatocytesis turned on by PMSG administration. FIG. 26C shows localization ofDRAGON mRNA in mouse testes by in situ hybridization. Bright (left) anddark (right) field images are shown. FIG. 26D shows immunolocalizationof DRAGON in d3 (D3, a-c) and d60 (D60, d-f) epididymis. Caput (a andd), corpus (b and e), and cauda (c and f) were dissected.

FIGS. 27A-27C are images showing cellular localization of DRAGON in themouse ovary and uterus by immunohistochemistry and in situhybridization. For immunohistochemistry, all sections were stained withDAB and counterstained with hematoxylin. Images are shown at lower (40)or higher (100) magnification. FIG. 27A shows DRAGON immunostaining inovaries at d60 (D60, a-d) and d9 (D9, f). The staining in oocytes ofsecondary follicle (arrow) is stronger than that in oocytes of antral(arrowhead) and atretic (curved arrow) follicles (a and d). DRAGON isnot expressed in oocytes of primordial (open arrow) and primary(triangle) follicles (b and c). For negative control, sections wereincubated with DRAGON antibody preincubated with competing immunizingpeptide (e). FIG. 27B shows localization of DRAGON mRNA in mouse adultovaries by in situ hybridization. Dark (b and d) and bright (a and c)field images are shown. Signals are confined to oocytes, and signals arestronger in the secondary (arrow) than in antral (arrowhead) follicles(a and b). No signals are seen in primary follicles (triangle, c and d)and in corpus luteurii (CL). FIG. 27C shows immunohistochemistry of themouse uterus showing protein expression of DRAGON (a and b), andnegative control (c), incubation with DRAGON antibody preincubated withcompeting immunizing peptide. The solid arrow indicates luminalepithelial cells of endometrium, the arrowhead indicates glandularepithelial cells of endometrium, and the open arrow shows circularmuscle.

FIGS. 28A and 28B are images showing immunolocalization of DRAGON in thepituitary and colocalization of DRAGON and FSH. FIG. 28A showsimmunostaining for DRAGON in the mouse pituitary (paraffin sections).Sections were incubated with DRAGON antibody (a-c) or with DRAGONantibody preincubated with competing immunizing peptide (d-f), AL,Anterior lobe; IL, intermediate lobe; PL, posterior lobe. FIG. 28B showscolocalization of FSH and DRAGON in the mouse anterior pituitary (frozensections). DRAGON and FSH were detected in the anterior pituitary.Double-labeled cells (arrows) indicate DRAGON expression in the mousepituitary gonadotrope. Note that some FSH-positive cells are negativefor DRAGON staining and some DRAGON-positive cells are negative for FSHstaining.

FIGS. 29A-29C are images showing expression of DRAGON in cell linesderived from reproductive tissues and localization of DRAGON into lipidrafts. FIG. 29A shows RT-PCR analyses of DRAGON mRNA in cell lines.13-Actin was used as a control for cDNA quality. FIG. 29B showsimmunochemical localization of DRAGON in Ishikawa cells. The liveunfixed cells were incubated with rabbit anti-DRAGON serum on ice andthen fixed in 2% paraformaldehyde. The bound antibodies were detected byincubating with FITC-conjugated donkey antirabbit IgG. FIG. 29C showslocalization of DRAGON in raft-enriched fractions prepared from Ishikawacells. Cells were extracted using a buffer containing 1% Triton X-100.The lysate was mixed with 85% sucrose, sequentially layered with 35 and5% sucrose, and centrifuged for 14 h at 150,000 g. Nine fractions werecollected and analyzed for DRAGON, caveolin-1, and β-actin by Westernblot. The bands specific for DRAGON are marked with asterisks.

FIGS. 30A-30D are graphs showing that DRAGON enhances cellular responseto BMPs in cell lines derived from reproductive tissues. FIG. 30A showsIshikawa or KGN cells were transiently transfected with BRE-Luc reporterin combination with increasing doses of DRAGON cDNA and assayed forluciferase activity. Transfection with DRAGON increases BRE-Luc responsein the absence of exogenous ligand. Values are ratios of BRE-Luc topRL-TK and are mean±SE of triplicates from representative experiments.DRAGON protein is detectable by Western blot in Ishikawa cells aftertransfection with 100 ng cDNA. The membrane was stripped and reprobedwith β-actin antibody as a control for loading (inset). FIG. 30B showsIshikawa cells transfected with BRE-Luc reporter and DRAGON cDNA andtreated with BMP2 alone or together with noggin. Values are ratios ofBRE-Luc to pRL-TK and are mean±SE of triplicates from representativeexperiments. FIG. 30C shows control medium used in parallel withnoggin-conditioned medium to demonstrate the specificity of theinhibitory activity of noggin-conditioned medium in BMP2 signaling.Although noggin inhibited BMP2 signaling, control conditioned medium hadno inhibitory effect. FIG. 30D shows Ishikawa cells transientlytransfected with BRE-Luc reporter and DRAGON (0 or 10 ng) in 24-wellplates were incubated with increasing doses (0-2800 pM) of BMP2. Valuesare fold increases of luciferase activity in treated cells relative tountreated cells and are the means SE of six determinations from threeindependent experiments in duplicate. Asterisks indicate significantdifferences between transfected and untransfected cells at each BMP2dose: **, P 0.01; *, P 0.05.

FIGS. 31A-31C are graphs showing RGMa signals via the BMP, but not theTGF-β, pathway. FIGS. 31A and 31B show LLC-PK1 cells transfected withthe BMP-responsive firefly luciferase reporter (BRE-Luc, A) or theTGF-β-responsive firefly luciferase reporter (CAGA-Luc, B), incombination with pRL-TK Renilla luciferase vector, either alone (A, Bbars 1, 2) or with 0.2 mg RGMa cDNA (A, B bar 3). Transfected cells werethen incubated for 16 hrs in the absence (A, B bars 1, 3) or presence of50 ng/ml BMP-2 (A, bar 2) or 20 ng/ml TGF-β1 (B, bar 2) followed bymeasurement of luciferase activity. FIG. 31C shows LLC-PK1 cellstransfected with BRE-Luc and pRL-TK either alone or with increasingamounts of RGMa cDNA as indicated. Transfected cells were then incubatedfor 16 hrs in the absence (white bars) or presence of 50 ng/ml BMP-2(black bars), followed by measurement of luciferase activity. Luciferasevalues were normalized for transfection efficiency relative to Renillaactivity to generate relative luciferase units (R.L.U.). Results arereported as the mean+/−standard deviation.

FIGS. 32A-32C are graphs and a gel image showing RGMa-mediated BMPsignaling is ligand-dependent. FIGS. 32A and 32B show LLC-PK1 cellstransfected with BRE-Luc and pRL-TK alone (A, B bars 1-2, 5-6) or incombination with 0.2 mg RGMa cDNA (A, B bars 3-4). RGMa transfectedcells were then incubated alone (A, B bar 3) or with 1 mg/ml nogginprotein (A, bar 4) or 20 mg/ml neutralizing antibody against BMP-2 andBMP-4 (α-BMP2/4, B, bar 4) for 48 hrs. As a control, cells without RGMawere incubated for 48 hrs in the absence (A, B, bars 1, 5) or presenceof 1 mg/ml Noggin protein (A, bars 2, 6) or 20 mg/ml α-BMP2/4 (B, bars2, 6), without (A, B, bars 1, 2) or with exogenous BMP-2 at 75 mg/ml (A,bars 5-6) or 25 mg/ml (B, bars 5-6) for 16 hrs. Luciferase activity wasmeasured from cell extracts and normalized for transfection efficiencyrelative. to Renilla activity to generate relative luciferase units(R.L.U.) Results are reported as the mean+/−standard deviation. FIG. 32Cshows RT-PCR performed on total RNA from LLC-PK1 cells using primers forBMP-2 or BMP-4 as indicated (lane 3). Purified plasmid cDNAs containingBMP-2 or BMP-4 were used as positive controls (+, lane 1), and reactionswithout template were used as negative controls (−, lane 2).

FIG. 33 is an image of a Western blot showing expression of solubleRGMa.Fc fusion protein. Soluble RGMa.Fc fusion protein was purified fromthe media of stably transfected HEK-293 cells via one-step Protein Aaffinity chromatography. Protein A purified media from cells transfectedwith empty vector was used as a negative control (Mock). Purifiedprotein was analyzed by reducing SDS-PAGE followed by Western blot withanti-RGMa peptide antibody (α-RGMa, right lane) or anti-human Fcantibody (α-Fc, left 2 lanes) as indicated.

FIGS. 34A and 34B are a graph and an autoradiography gel image showingsoluble RGMa.Fc binds BMP-2 and BMP-4 selectively. FIG. 34A shows400,000 counts ¹²⁵I-BMP-2 was incubated overnight alone (background) orin combination with 25 ng RGMa.Fc, in the absence (total binding) orpresence of excess unlabeled BMP-2, -4, -7, or TGF-β1 as indicated,followed by incubation on protein A coated plates and determination ofradioactivity using a standard g counter. FIG. 34B shows buffer alone(C), 25 ng RGMa.Fc, or TGF-β type I receptor ALK5.Fc incubated with400,000 counts ¹²⁵I-BMP-4 with or without excess cold BMP-4 overnight at4° C. This mixture was then incubated in the absence (−DSS) or presenceof 2.5 mM DSS in DMSO (+DSS) as indicated for 2 hr at 4° C. Afterquenching of DSS activity, the mixture was incubated with Protein Abeads at 4° C. for 2 hr, and the eluted protein complex analyzed bynon-reducing SDS-PAGE, followed by autoradiography.

FIGS. 35A-35C are graphs and images of Western blots showing RGMamediates BMP signaling through BMP receptors. FIG. 35A shows, in theLeft panel, LLC-PK1 cells transfected with BRE-Luc and pRL-TK eitheralone, (bar 1) or in combination with 0.2 mg RGMa cDNA (bars 2-4), inthe absence (bar 2) or presence of 1 mg dominant negative type Ireceptors ALK3 (ALK3 DN, bar 3) or ALK6 (ALK6 DN, bar 4). In the Rightpanel, LLC-PK1 cells were transfected with BRE-Luc and pRL-TK eitheralone (bars 5,6) or in combination with 1 mg ALK3 DN (bar 7) or ALK6 DN,(bar 8), followed by incubation in the absence (bar 5) or presence of 50ng/ml BMP-2 (bars 6-8). Luciferase activity was measured from cellextracts and normalized for transfection efficiency relative to Renillaactivity to generate relative luciferase units (R.L.U.). Results areexpressed as the mean+/−standard deviation. FIG. 35B shows 200 ngRGMa.Fc, 200 ng ALK6.Fc, and/or 100 ng BMP-2 incubated in solution invarious combinations as indicated with the crosslinker DSS. Complexeswere pulled down with protein A beads, and the protein complex wasanalyzed by non-reducing SDS-PAGE followed by Western blot with RGMaantibody. Arrowheads indicate slower migrating bands containing RGMa.Fccomplexes. FIG. 35C shows buffer alone (bar 1), 10 ng RGMa.Fc alone (bar2), 10 ng ALK6.Fc alone (bar 3), or the combination of 10 ng each ofRGMa.Fc and ALK6.Fc (bar 4) incubated with ¹²⁵I-BMP-2, followed byincubation on protein A coated plates and determination of radioactivityusing a standard g counter.

FIGS. 36A-36D show RGMa mediates BMP signaling through Smad1/5/8 andupregulates Id1. FIG. 36A shows LLC-PK1 cells transfected with BRE-Lucand pRL-TK either alone (lane 1, 7-8), or in combination with 1 mgwildtype Smad1 (Smad1 WT, bar 2,9) or 1 mg dominant negative Smad1(Smad1 DN, bar 3, 10). Transfected cells were then incubated in theabsence (bar 1-3, 7) or presence of 50 ng/ml BMP-2 (bars 8-10).Alternatively, cells were co-transfected with BRE-Luc, pRL-TK, and 0.2mg RGMa alone (bar 4), or in combination with Smad1 WT (bar 5) or Smad1DN (bar 6). Luciferase activity was measured from cell extracts andnormalized for transfection efficiency relative to Renilla activity togenerate relative luciferase units (R.L.U.) Results are reported as themean+/−standard deviation. FIG. 36B shows LLC-PK1 cells transientlytransfected with 5 mg RGMa cDNA (middle 3 lanes) or empty vector (leftand right 2 lanes). 24 hours after transfection, cells were incubatedwithout (lanes 1-5) or with 50 ng/ml BMP-2 (right two lanes) for twohours. Cell lysates were analyzed by Western blot in succession withRGMa antibody (α-RGMa), phosphorylated Smad1/5/8 antibody(α-p-Smad1/5/8), Smad1 antibody (α-Smad1, as a loading control), Id1antibody (α-Id1), and actin antibody (α-β-actin, as a loading control).FIGS. 36C and 36D show chemiluminescence from the Western blot in panelB quantitated by IPLab Spectrum software for phosphorylated Smad1/5/8relative to Smad1 expression (FIG. 36C) and Id1 relative to β-actinexpression (FIG. 36D). Results are reported as the mean+/−standarddeviation of control cells (C), cells transfected with RGMa (RGMa), andcells treated with BMP-2 (BMP-2).

FIG. 37 is a Northern blot of RGMa expression in adult rat tissues. 10mg total RNA was loaded per tissue per lane and probed for RGMa. Tissuesare indicated by name.

FIGS. 38A-38F are images of immunohistochimcal sections showing RGMa andnuclear phosphorylated Smad1/5/8 expressed in ventral horn motor neuronsof adult rat spinal cord. Fixed adult rat spinal cord sections wereco-immunostained with rabbit anti-RGMa antibody (α-RGMa, FIGS. 38A-38C)or rabbit anti-phosphorylated Smad1,5,8 antibody (α-p-Smad1/5/8; FIGS.38D-38F) in combination with mouse anti-neuron-specific nuclear proteinantibody to visualize neuronal cell bodies (α-NeuN, all panels),followed by Cy3-conjugated anti-rabbit and FITC-conjugated anti-mousesecondary antibodies. Shown are images of the ventral horn byfluorescence microscopy. Cy3 fluorescence is shown in the left column ofpanels (α-RGMa (FIG. 38A) or α-p-Smad1,5,8 (FIG. 38D)). FITCfluorescence is shown in the middle column of panels (α-NeuN (FIGS. 38Band 38E)). The corresponding superimposed images are shown in the rightcolumn of panels (Merge (FIGS. 38C and 38F)). Representative motorneurons are indicated (arrows).

FIGS. 39A and 39B are a Western blot and an immunocytochemical analysisshowing increase in levels of phosphorylated-Smad1 (pSmad1) in culturedDRG treated with BMP2. Note that levels of Smad1 are unchanged.β-tubulin levels were used as loading Control. Immunocytochemicalanalysis FIG. 39B shows induction of phospho-Smad1 in cultured DRGneurons After BMP2 treatment (bottom) as compared to control (top).

FIGS. 40A and 40B are images showing DRG neurons cultured (48 hrs) inthe absence (FIG. 40A; Cont) or presence of BMP2 (100 ng/ml; FIG. 40B)and stained for β-tubulin III to assess neurite outgrowth. Note theincrease in neurites after incubation with BMP2 indicating that BMP2acts directly on sensory neurons to induce neurite outgrowth.

FIGS. 41A-41G are a schematic diagram and images showing results from anexperiment similar to the one described in FIGS. 39 and 40, using thespinal cord slice paradigm (FIG. 41A) to show that spinal cord neuronsalso respond to BMP2 via the Classical Smad1,5,8 signaling pathway(FIGS. 41B-41G). After BMP2 treatment (100 ng/ml), the slices wereanalyzed by immunohistochemistry to assess levels of phosphorylatedSmad1 (p-Smad1) as a marker for BMP signaling.

FIG. 42 is an image showing the slices co-stained with anti-NeuN (anantibody that labels neurons) to show that the detected increase of BMPsignaling (phospho-Smad1) observed in the spinal cord is also neuronal.

DETAILED DESCRIPTION The TGF-β Signaling Pathway

Signaling through the TGF-β pathways influences the growth anddifferentiation of a variety of cell types by affecting genetranscription. TGF-β ligands, the extracellular signaling molecules,induce the formation of functional receptor complexes by combiningmembers of two distinct families of serine/threonine kinases; the Type Iand Type II TGF-β receptors. The Type II receptor activates, byphosphorylation, the Type I receptor which propagates the TGF-β signalby phosphorylating a member of the cytoplasmic receptor-activated Smad(R-Smad) protein family. Each TGF-β ligand has the capability toassemble several possible Type I/Type II receptor combinations anddifferent combinations phosphorylate different intracellular R-Smadprotein substrates. Signaling by an Activin/Nodal TGF-β ligand typicallyresults in the phosphorylation of either Smad2 or Smad3; whereas BMP/GDFTGF-β ligand signals are frequently transduced through Smad1, Smad5, orSmad8. The R-Smads form heteromeric complexes with the Co-Smads andSmad4, and then translocate from the cytoplasm into the nucleus toregulate gene transcription.

The TGF-β ligands BMP2, BMP4, BMP7, and GDF5 activate the BMP/GDF branchof the TGF-β signaling pathway. Upon ligand binding, a functional TGF-βreceptor is formed from BMP type I receptor (BMPRI) and a BMP type IIreceptor (BMPRII). The BMPRIs that mediate BMP2, BMP4, and BMP7signaling include ALK2, ALK3, or ALK6. The group from which the BMPRIIis selected includes the prototypical BMP type II receptor (BMPRII), theActivin type IIA receptor (ActRIIA), and the Activin type IIB receptor(ActRIIB)

Multiple extracellular and intracellular regulators enhance or reduceTGF-β and BMP signaling. Access of ligands to receptors is inhibited bysoluble proteins that bind and sequester the ligand. These include LAP,decorin, and α2-macroglobulin, which bind to TGF-β. Soluble BMPantagonists include noggin, chordin, chordin-like, follistatin, FSRP,the DAN/Cerberus protein family, and sclerostin.

TGF-β ligand access to receptors is also controlled by membrane-boundco-receptors. The proteoglycan beta-glycan (TGF-β type III receptor)enhances TGF-β binding to the type II receptor while endoglinfacilitates binding to Alk1. The connective tissue growth factor (CTGF)enhances TGF-β and inhibits BMP4 receptor binding. The CFC-EGF familyregulates TGF-β signaling both as secreted factors and cell surfacecomponents. Cripto, a GPI-anchored membrane protein, increases bindingof nodal, Vg1, and GDF1 to activin receptors but inhibits activinsignaling by forming an “inert” complex with activin and ActRII. BAMBIacts as a decoy receptor that competes with the type I receptor forincorporation into ligand-induced receptor complexes.

So far only co-receptors that act on the TGF-β/activin/nodal signaltransduction pathway have been identified. We have discovered thatDRAGON, a membrane-associated GPI-anchored protein that is expressedearly in vertebrate embryos, binds to BMP but not other TGF-β ligands,associates with BMP receptors, and activates BMP signaling in cell linesand Xenopus embryos. We conclude that DRAGON is the first identifiedco-receptor that enhances BMP signaling and may be used to modulate theBMP/GDF branch of the TGF-β signaling pathway.

Characterization of DRAGON

Murine DRAGON (SEQ ID NOs: 1 and 5) was identified, using a GenomicBinding Site (GBS) strategy, as a seminal member of a new family ofDRG-11 responsive genes that are involved in embryogenesis. DRAGONhomologs have been identified in the human (SEQ ID NOs: 2 and 6),Zebrafish (SEQ ID NOs: 4 and 7), and C. elegans (SEQ ID NO: 3).

Sequence analysis of the mDRAGON coding region identified conserveddomains with homology to notch-3,phosphatidylinositol-4-phosphate-5-kinase type II beta), insulin-likegrowth factor binding protein-2, thrombospondin, ephrin type-B receptor3 precursor, and Slit-2, all of which are known to influence axonalguidance, neurite outgrowth, and other neuronal developmental functions.The C-terminus of mDRAGON is also predicted to contain a hydrophobicdomain indicative of a 21 amino acid extracellular GPI anchoring. Acomputational structure-function analysis of mDRAGON reveals thepresence of a putative signal peptide sequence (FIG. 1), indicating thatthe gene product is a secreted protein, and further supporting anextracellular localization.

DRAGON Protein Expression

A rabbit polyclonal antibody was raised against the peptide sequenceTAAAHSALEDVEALHPRK (SEQ ID NO: 8; residues 388-405 of mDRAGON), presentin the C-terminus of DRAGON, upstream of its hydrophobic tail. Theantibody binds with high affinity to recombinant DRAGON expressed inHEK293T transfected cells, recognizing a band of 50 KDa in Western blots(FIG. 2 a). Antibody specificity was confirmed by immunocytochemistry ofDRAGON-expressing HED293T cells (FIG. 2 b). Western blots of proteinextracts from neonatal and adult DRG and DRG primary cultures show asimilar band with an additional lower band of 40 KDa, indicatingpossible proteolytic cleavage of endogenous DRAGON. Treatment of HEK293Tcells expressing DRAGON with PI-PLC results in the decrease of DRAGONdetection on HEK cells and its release into the culture medium (FIG. 2c), indicating that DRAGON is GPI-anchored.

Immunohistochemistry confirms expression of DRAGON in the DRG, spinalcord and brain in the areas where DRAGON mRNA is found (FIG. 2 d). Inthe adult DRG, DRAGON is more abundantly expressed in small neurons withunmyelinated axons than in medium and large myelinated neurons (Aδ andAβ-fibers) (FIG. 2 d). In the adult spinal cord, DRAGON expression ismost prominent in the superficial laminae of the dorsal horn (FIG. 2 d).Immunohistochemical studies also demonstrated that the DRAGON protein isexpressed in the E14.5 mouse retina and optic nerve (FIG. 3) and skin(FIG. 4).

Tissue Localization of DRAGON Gene Expression

Initial studies using in situ hybridization demonstrated that, at E12.5,DRG11 and DRAGON expression overlaps (FIG. 5 a). In the DRG most neuronsexpress both DRG11 and DRAGON; in the spinal cord DRG11 and DRAGON areexpressed in the same medial region adjacent to the ventricular zone(FIG. 5 a). A pull down assay was carried out to confirm interaction ofDRG11 with the 363 by promoter fragment of DRAGON obtained with the GBSscreening. The promoter fragment was pulled down by a GST-DRG11-DBDfusion protein but not GST (FIG. 5 c). Finally, DRAGON mRNA expressionin DRG11 null mutant embryonic mice was examined. DRAGON expression inthe spinal cord and DRG were significantly reduced in DRG11^(−/−) micecompared to wildtype littermates (FIG. 5 d). DRAGON mRNA is alsoexpressed in embryonic and adult mouse DRGs, spinal cord, and brain,with little or no expression in the liver and kidney, and low levels inthe heart (FIGS. 5 e, 6, and 7).

DRAGON is expressed throughout vertebrate embryonic development (FIG. 5f) but primarily in the developing peripheral and central nervoussystems. It's expression begins in early embryogenesis, in ES cells andE2.5 embryos and in oocytes at the germinal vesicle stage indicates thatDRAGON expression is maternal (FIG. 8). Post-implantation mouse embryos(>E7) also express DRAGON protein and mRNA (FIGS. 8A and 8B). In E10.5mouse embryos, DRAGON expression is along the neural tube, in the dorsalroot ganglia, and, at maximum levels, in the tips of the neural foldsand the tail bud (FIG. 8C). The pattern of DRAGON expression is similarto that of the BMP receptors, particularly the type I receptors ALK3 andALK6, and the type II receptor BMPRII (Dewulf et al., 1995; Soderstromet al., 1996). The expression of the Xenopus orthologs of DRAGON andmRGM at different developmental stages of Xenopus leavis embryos shows adorsal (e.g. roofplate) gradient-like pattern (FIGS. 8D and 8E) and isalso consistent with that of members of the TGF-β superfamily signalingpathway. These observations prompted us to investigate if DRAGONcontributes to or modulates TGF-β superfamily signal transduction.

DRAGON Enhances BMP but Not TGF-fi Intracellular Signaling

In order to investigate the effect of DRAGON on BMP signaling, aBMP-responsive luciferase reporter construct (BRE-Luc; Korchynskyi etal., 2002) was transfected, with or without a DRAGON expressionconstruct, into LLC-PK1 (kidney epithelial cell line), HepG2, or 10T1/2cells. Stimulation of the LLC-PK1 cells with BMP2 (50 ng/ml) caused a4-fold increase in luciferase activity compared to control (FIG. 9A).This effect was mimicked by the co-transfection and expression ofDRAGON, in the absence of exogenous BMP-2.

The stimulatory effect of DRAGON was not observed for all TGF-β ligands.LLC-PK1 cells were transfected with a TGF-β-responsive luciferasereporter construct ((CAGA)12-MLP-Luc; Dennler et al., 1998). These cellsdemonstrated a 12-fold increase in luciferase activity upon stimulationwith 10 ng/ml TGF-β1, but no effect was observed following theco-transfection of DRAGON without TGF-β1 stimulation (FIG. 9A).Expression of ‘DRAGON in transfected LLC-PK1 cells was confirmed byWestern blot (data not shown).

To assess whether DRAGON expression dynamically regulates BMP signaling,LLC-PK1 cells were co-transfected with the BRE-luc reporter constructand increasing amounts of a DRAGON cDNA expression vector (2 and 20 ng).Enhanced luciferase expression was measured following BMP2 stimulationof DRAGON-expressing cells compared to the untransfected controls.Specifically, 50 ng/ml BMP2 induced a 14-fold increase in luciferaseactivity in DRAGON co-transfected cells compared to a 6-fold increase inthe absence of DRAGON (FIG. 9B).

In order to confirm the effect of DRAGON on BMP-dependent signaling,10T1/2 and HepG2 cells were transfected with a luciferase reporter geneunder the control of another BMP-responsive promoter, Msx2-Luc.Confirming the previous studies, co-expression of DRAGON induced a4-fold increase in BMP-dependent signaling in the absence of anexogenously added BMP ligand (FIG. 9B). BMP signaling inDRAGON-expressing cells was increased 8-fold at a very low dose (50 pM)of exogenous BMP2 (FIG. 9B).

Results from the 10T1/2 and HepG2 cells also confirm that the effect ofDRAGON is specific to BMP signaling. Co-transfection of DRAGON withluciferase constructs responsive to TGF-β, Activin, or GDF8-inducedsignaling did not induce reporter gene expression, alone or in aligand-dependent manner (FIG. 9C and data not shown). These datademonstrate that DRAGON expression activates BMP but not TGF-β signalingand that DRAGON enhances ligand-mediated BMP signaling.

DRAGON Binds to BMP Type I and BMP Type II Receptors

The previous studies demonstrate that DRAGON enhances BMP-mediatedsignaling, but do not give an indication whether the effect occursthrough a direct modulation of BMP receptors or thought an independentreceptor pathway that converges on the Smad. To explore thesepossibilities we investigated first whether DRAGON interacts directlywith BMP receptors, second, the mechanism by which DRAGON activates BMPsignaling, and third, whether DRAGON binds to members of the TGF-βsuperfamily.

To test whether DRAGON interacts with BMP receptors, DRAGON wasco-transfected with type I or type II BMP receptors. The physicalinteraction between the receptors and DRAGON was studied byimmunoprecipitation using an anti-DRAGON antibody and probing withanti-ALK receptor antibodies tagged with a detectable HA.Immunoprecipitates from doubly transfected HEK 293 cells demonstratethat DRAGON interacts with ALK2, ALK3 and ALK6 (FIG. 10A). BMPRIIA andBMPRIIB also interact with DRAGON when coexpressed in HEK cells (FIG.10A). These data indicate that DRAGON has the ability to physicallyinteract with both BMP type I and type II receptors.

Dominant negative isoforms of the BMP type I receptor ALK6 and theintracellular effector Smad1 were each cotransfected with DRAGON toconfirm that DRAGON's action occurs via the classical BMP signalingpathway (FIGS. 10B and 10C). The dominant negative ALK6-KR mutant isdeficient in kinase activity and unable to phosphorylate the Smads(Faber et al., 2002). Co-expression of DRAGON with ALK6-KR abolished theDRAGON-mediated induction of BMP-luciferase activity (FIG. 8B).Similarly, co-expression of DRAGON with a Smad1 dominant negative mutantthat lacks the carboxy-terminal phospho-acceptor domain (Shi et al.,2003) reduced dose dependently DRAGON-induced signaling to baselinelevels, whereas co-expression of wild type Smad1 enhanced this signaling(FIG. 10C). These data indicate that DRAGON binds to BMP receptors andutilizes the Smad effectors to activate and enhance the BMP-signaltransduction pathway.

DRAGON Enhances BMP Signaling in a Ligand-dependent Manner

The expression of DRAGON, in the absence of a BMP ligand, leads toactivation of BMP signaling (FIG. 9A). Likewise, DRAGON enhancesBMP-dependent gene expression in the presence of BMP ligand. To furtherstudy the contribution of DRAGON to the BMP signaling cascade,DRAGON-activated signaling was measured in the presence of the welldocumented BMP antagonist noggin (Balemans et al., Dev. Biol. 250:231-250, 2002). When added to HepG2 cells, noggin inhibited DRAGON orBMP2 induced activation of the BRE-Luc reporter gene construct in a dosedependent manner (FIG. 11A), but had no effect on activin stimulation ofthe CAGA reporter construct (data not shown). Furthermore, equal dosesof follistatin, sufficient to block activin activity, had no significanteffect on DRAGON or BMP2 activity in the BRE-Luc-expressing cells (FIG.11B), demonstrating that the noggin effect on DRAGON signaling isspecific. These results also suggest a role for endogenous BMP typeligand(s) in this system.

Noggin is known to exert its inhibitory effect on BMP signaling througha direct binding and neutralization of the BMP ligands. The possibilityof a direct interaction between noggin and DRAGON was assessed in orderto evaluate noggin's effect on DRAGON-enhanced signaling. Increasingamounts of the purified soluble fusion protein DRAGON-Fc (Samad et al.,2004) were incubated with a fixed amount of radio-labeled [¹²⁵I]-nogginfollowed by co-precipitation with protein A. No significant interactionbetween [¹²⁵I]-noggin and the DRAGON-Fc could be detected when comparedto no-protein controls (FIG. 11C). An anti-noggin antibody, as apositive control, detected substantial quantities of [¹²⁵I]-noggin,demonstrating that the labeled noggin was intact (FIG. 11C). Theseresults were confirmed in a similar assay using COS-7 cells in which nosignificant association between the two molecules was observed (FIG.11D). These observations prove that noggin does not directly bind toDRAGON and that the DRAGON-mediated activation of BMP signaling isligand dependent.

DRAGON Binds to BMP but not TGF-β Ligands

HEK 293 cells were transiently transfected with the DRAGON expressionconstruct, and the binding of either [¹²⁵I]-BMP2 or [¹²⁵I]-TGF-β to thetransfected cells was determined. As shown in FIG. 12A, [¹²⁵I]-BMP2affinity-labeled the DRAGON-expressing cells, but [¹²⁵I]-TGF-β did not.Hemaglutinin-tagged ALK6 (a BMP type I receptor) and TGF-receptor typeII (TβRII) expression constructs were used as positive controls for[¹²⁵I]-BMP2 or [¹²⁵I]-TGF-β binding, respectively. Immunoprecipitationusing an anti-HA antibody confirmed binding of BMP2 and TGF-β to ALK6and TβRII respectively (FIG. 12A).

The binding affinity of the soluble DRAGON-Fc fusion protein (Samad etal., 2004; Del Re et al., 2004) to [¹²⁵I]-BMP2 was studied in thepresence of increasing amounts of unlabeled BMP2 (FIG. 12B). DRAGON-Fcbinds [¹²⁵I]-BMP2 with high affinity and an apparent dissociation rateconstant (Kd) of 1.5 nM (FIG. 12B). The binding of [¹²⁵I]-BMP2 to DRAGONwas inhibited by the addition of excess unlabeled BMP2 as well asunlabeled BMP4 (4 nM) (FIG. 12C). However, the BMP2-DRAGON interactionwas not disrupted by 4 nM BMP7, or 1 nM Activin A, TGF-β1, TGF-β2, orTGF-β3 (FIG. 12C).

To further confirm the ability of DRAGON to bind to BMP2 and BMP4, wetested whether increasing amounts of exogenous DRAGON-Fc cancompetitively inhibit BMP-mediated signaling. Pretreatment of HEK 293cells with increasing doses of DRAGON-Fc (60 and 300 ng/ml) leads, in adose dependent manner, to a reduction of BMP2 mediated activation ofBRE-Luc promoter as assessed by luciferase activity (FIG. 12D). However,as predicted from the binding experiments, DRAGON-Fc was not able tocompete and inhibit BMP7 or TGF-β1 signaling (CAGA-Luc promoter was usedto assess TGF-β signaling; FIG. 12E).

These data indicate that DRAGON interacts with members of the BMP ligandfamily and with BMP receptors to enhance intracellular BMP signaling.Because DRAGON binds both the BMP ligands and receptors, it is thereforea component of the BMP receptor complex.

DRAGON Enhances BMP Signaling when Expressed Only on the Cell Surface

As described above, DRAGON is normally a membrane bound protein. To testwhether membrane anchoring is essential to its effect on BMP signaling,a DRAGON-Fc construct was created in which the C-terminal GPI anchor wasdeleted and replaced by the human Fc domain. When expressed, theDRAGON-Fc was secreted into the culture medium, whereas wild-type DRAGONis directed (at least in part) to the plasma membrane. The DRAGON-Fc,when co-transfected with the BRE-Luc reporter construct, failed toenhance BMP signaling (FIG. 13A). The expression of both DRAGON andDRAGON-Fc was confirmed by Western blot (FIG. 13B). Thus, these resultsdemonstrate that, in order for DRAGON to function as a cell surfaceco-receptor for BMPs, it must be inserted into the plasma membrane. FIG.14 conceptually outlines the location and function of DRAGON as a BMPco-receptor.

DRAGON Enhances BMP Signaling in Xenopus Embryos

Genetic evidence has shown that members of the BMP ligand family playpivotal roles in the gastrulation of mouse embryo, a process that laysdown the future body plan (Lu et al. 2001). BMPs regulate theproliferation, survival, and patterning of the epiblast; the inductionof primordial germ cell precursors; and formation of the mesoderm(Mishina et al. 1995; Winnier et al. 1995; Lawson et al. 1999). Mutantembryos deficient in BMP4 or BMPRIA are blocked at the beginning ofgastrulation, and fail to form mesoderm (Ying and Zhao 2001). BMPs werealso shown to be involved in endoderm formation (Kondoh et al., 2003).In order to investigate the contribution of DRAGON expression to BMPsignaling and function in vivo, mDRAGON was expressed in Xenopus embryosand the expression of inesodermal and endodermal markers was assessed.

Mouse DRAGON mRNA was injected into Xenopus embryos at gastrula stagesto investigate whether DRAGON alters BMP signaling, by measuring itsinteraction with Smad1. DRAGON co-expression with Smad1 led to inductionof mRNA for the pan-mesodermal marker Xbra and two endodermal markersmix 1 and mixer mRNAs (FIG. 15A). At the doses used, DRAGON or Smad1alone did not significantly induce expression of these markers (FIG.15A). The reduction in the activity threshold of Smad1 by DRAGON iscompatible with an enhancement in BMP signaling, as detected in the cellbased assays.

DRAGON Regulates Neural Induction in Xenopus Embryos

In order to determine whether DRAGON affects cell differentiation andearly embryonic development, DRAGON was injected into one cell at theanimal pole of Xenopus embryos at the 2-cell stage. Embryos were allowedto develop until early tadpole stages. By injecting one out of twocells, a control side and an experimental side are present in the sameembryo. A variety of markers were measured, including twist (expressedin anterior neural crest cells) and N-tubulin (a general neuronaldifferentiation marker), to determine whether DRAGON affects earlyneural patterning. Ectopic DRAGON caused a decrease in neural crestderivatives, as shown by loss of twist expression (FIG. 15C, top panels)and an increase in neuronal markers (FIG. 15C, bottom panels).

In ectodermal explant assays, DRAGON induced anterior neural markers(FIG. 15B). Nrp1 is a pan-neural marker, Otx2 is expressed within theforebrain and midbrain regions, and XAG is expressed in the cement gland(the most anterior structure in the tadpole). In addition, DRAGONinduced nkx2.5, an early marker of cardiac development.

DRAGON Promotes Neuronal Survival

The anti-DRAGON polyclonal antibody was added to neonatal rat DRGneuronal cultures to investigate the contribution of DRAGON to neuronalsurvival. Neuronal cultures were treated with 0.25% anti-DRAGON serum,0.25% pre-immune serum (negative control), or vehicle. A statisticallysignificant 20-25% increase in neuronal cell death was measuredfollowing anti-DRAGON treatment compared to controls.

0.25% anti- 0.25% DRAGON pre-immune Vehicle Control serum serum (noserum) % viable neurons 41.8% 55.3% 51.8% (mean) Standard Error (S.E.) 1.7%  2.3%  2.5% Number of isolated 12 12 11 DRG cultures (n)

Localization and Action of DRAGON (Repulsive Guidance Molecule b) in theReproductive Axis

The importance of DRAGON in reproduction is indicated by its pattern ofexpression in reproductive tissues. Mammalian reproduction is regulatedby endocrine hormones such as pituitary FSH and LH as well as by locallyproduced growth factors, including TGF-β superfamily members activin,inhibin, and bone morphogenetic proteins (BMPs) (Welt et al., Exp BiolMed (Maywood) 227:724-752, 2002). BMPs were originally identified bytheir ability to induce bone and cartilage formation (Wozney et al.,Science 242:1528-1534, 1988). However, numerous studies have revealedthat BMPs have a wide variety of effects on many cell types, includingmonocytes and epithelial, mesenchymal, and neuronal cells, and playpivotal roles in cytodifferentiation, morphogenesis, and organogenesis(Kawabata et al., Cytokine Growth Factor Rev 9:49-61, 1998). Members ofthe TGF-superfamily, including BMPs, transduce their signals throughbinding to type I and II serine/threonine kinase receptors. BMPsignaling is mediated intracellularly by the phosphorylation ofreceptor-activated Smads (R-Smads) 1, 5, and 8. Activated R-Smadscomplex with the common partner Smad 4 and translocate to the nucleuswhere they initiate BMP-stimulated alterations in target geneexpression. Signaling of TGF-superfamily members including BMPs is alsomodulated by soluble extracellular proteins such as noggin, chordin, andgremlin. In addition, membrane-associated proteins, including betaglycan(TGF-type III receptor), endoglin, and crypto are also critical forassisting with ligand binding to receptor or for altering receptorspecificity (reviewed in Massague et al., Genes Dev 14:627-644, 2000;Shi et al., Cell 113:685-700, 2003; Derynck, R. Zhang Y E, Nature425:577-584, 2003).

The mRNAs encoding BMP2, 3, 3b, 4, 6, 7, and 15 have been identified inmammalian ovaries. Moreover, BMP receptors BMPRIA, IB, and II are widelyexpressed in the ovary, with the strongest expression in the granulosacells and oocytes of developing follicles in normally cycling rats(Shimasaki et al., Proc Natl Acad Sci USA 96:7282-7287, 1999; Shimasakiet al., Endocr Rev 25:72-101, 2004). BMPs and their receptors are alsoexpressed in uterine stroma and glandular epithelium (Erickson G F etal., J Endocrinol 182:203-217, 2004). In males, BMP2, BMP4, and BMP8Aand BMP8B are expressed in germ cells, and BMP4, BMP7, and BMP8A areexpressed in the epididymis (reviewed in Shimasaki et al., Endocr Rev25:72-101, 2004). Moreover, mice deficient in BMP4, BMP8A, or BMP8B showgerm cell degeneration in the testis and/or epithelial cell degenerationin the epididymis (Hu et al., Dev Biol 276:158-171, 2004; Zhao et al.,Genes Dev 10:1657-1669, 1996; Zhao et al., Development 125:1103-1112,1998). Together, these results suggest that BMPs may play importantroles in regulating reproduction.

DRAGON was identified through a genomic screening strategy for genesregulated by DRG11, a homeobox transcription factor that is expressed inembryonic dorsal root ganglion (DRG) (Samad et al., J Neurosci24:2027-2036 2004). Independently, this gene was also cloned as RGMb,one of three mouse homologues of the chicken repulsive guidance molecule(RGM) (Schmidtmer et al., Gene Expr Patterns 4:105-110 2004). The DRAGONgene encodes a 436-amino-acid glycosylphosphatidylinositol(GPI)-anchored protein, suggesting it may be associated with lipid raftswithin the plasma membrane. Indeed, adhesion of DRG neurons to HEK293cells was increased after transfection of HEK293 cells with DRAGON cDNA(Samad et al., J Neurosci 24:2027-2036 2004). DRAGON is expressed in anumber of neural tissues including embryonic and adult mouse DRGs,spinal cord, and brain (Samad et al., J Neurosci 24:2027-2036 2004,Niederkofler et al., J Neurosci 24:808-818 2004; Oldekamp et al., GeneExpr Patterns 4:283-288; 2004). Interestingly, DRAGON is also involvedin BMP signaling because 1) injection of DRAGON mRNA into Xenopusembryos induced expression of a number of BMP-regulated genes, 2) DRAGONbinds directly to BMP2, BMP4, and BMP receptors, and 3) transfection ofDRAGON cDNA into BMP-responsive cells enhances transcription of aBMP-responsive reporter (Samad et al., J Biol Chem 280:14122-14129,2005). These observations indicate that DRAGON acts as a BMP coreceptorthat regulates cellular response to BMP signals.

To understand the potential role of DRAGON in BMP signaling within thereproductive tissues, DRAGON expression in murine reproductive tissuesand cell lines was examined. DRAGON is expressed and dynamicallyregulated in gonadal germ cells and in epithelial cells of thereproductive tract including epididymis and uterus. DRAGON is alsoexpressed in the pituitary. As predicted from its being anchored to thecell membrane by a GPI anchor, DRAGON is indeed localized in lipid raftswhere it enhances BMP2 and -4 signaling. The overlapping expression andfunction of BMPs in the reproductive system indicates that DRAGON playsan important role in reproduction through enhancement of BMP signaling.

Expression of DRAGON mRNA in Reproductive Organs

DRAGON expression in mouse reproductive tract tissues was examined byRT-PCR (FIG. 25). DRAGON mRNA was detected in the testis, epididymis,and seminal vesicles in males and in the ovary, uterus, and pituitary infemales.

Cellular Localization of DRAGON in the Testis and Epididymis

Immunohistochemical analyses of dl and 3 mouse testes showed that DRAGONwas localized to gonocytes both in the center and at the basementmembrane of seminiferous tubules (FIG. 26A, a and b). In testes from d9mice, spermatogonia at the basement membrane were positive for DRAGONexpression (FIG. 26A, c). However, DRAGON staining in spermatogoniabecame much weaker in testes from 21 d-old mice (FIG. 26A, d).Interestingly, a few gonocytes, which remain in the central region ofthe tubules from d21 testes, were strongly stained with DRAGON (FIG.26A, d). Some interstitial cells showed weak staining in d1 testes (FIG.26A, a), but no staining was observed in interstitial cells in oldertestes. In adult (d60) testis, DRAGON was expressed in spermatocytes andround spermatids, whereas spermatogonia and Sertoli cells did not appearto express DRAGON (FIG. 26A, e and f). The staining in gonocytes,spermatogonia, and spermatocytes was completely abolished when theantiserum was preincubated with the competing immunizing peptide,demonstrating the specificity of the antiserum (FIG. 26A, g and h).DRAGON expression in spermatocytes and round spermatids from adulttestes was confirmed by in situ hybridization (FIG. 26C).

Spermatocytes of d21 testes were not stained with DRAGON antibody,whereas those cells of adult testes were strongly stained (FIG. 26A, dand e). Interestingly, DRAGON was highly expressed in spermatocytes oftestes collected after 2d of PMSG administration to 19d-old mice (FIG.26B), suggesting that DRAGON expression levels are hormonally regulated.

In d3 epididymis, DRAGON protein was found on both the apical and basalsides of epithelial cells with staining in the apical side strongercompared with the basal side. DRAGON staining was stronger in caudalthan in caput or corpus epididymis of 3d-old mice (FIG. 26D, a-c). Incontrast, DRAGON expression was primarily localized to the apical sideof epithelial cells of adult epididymis, and it appeared that DRAGON wasmore highly expressed in caput or corpus, compared with caudalepididymis (FIG. 26D, d-f). These results suggest that DRAGON may beinvolved in regulation of spermatogenesis and epididymal epithelialfunction.

Cellular Localization of DRAGON in the Ovary and Uterus

Within the adult mouse ovary, DRAGON protein was detected exclusivelywithin oocytes (FIG. 27A, a, b, and d) and was more intense in oocytesfrom secondary follicles compared with antral follicles (FIG. 27A, a).In contrast, no DRAGON staining was found in oocytes of primordial (FIG.27A, b) or primary (FIG. 27A, c) follicles, nor in somatic cells of anyfollicles (FIG. 27A). In atretic follicles, oocytes showed weak DRAGONstaining (FIG. 27A, d). There was no staining of the ovarian surfaceepithelium (FIG. 27A, b-d). Oocyte staining was completely blocked bypreincubating antiserum with competing immunizing peptide (FIG. 27A, e).In d9 ovaries, DRAGON staining was detected only in the oocytes ofsecondary follicles but not in the primordial or primary follicles (FIG.27A, f). PMSG treatment had no effect on DRAGON staining in oocytes(data not shown).

Consistent with immunostaining, DRAGON mRNA, as detected by in situhybridization, was stronger in oocytes from secondary follicles (FIG.27B, arrows) compared with antral (FIG. 27B, arrowheads) follicles andwas undetectable in oocytes from primary follicles (FIG. 27B, triangle).DRAGON mRNA was not detectable in ovarian somatic cells. These resultssuggest that DRAGON regulates the development of oocytes and folliclesby influencing the interaction between the oocyte and granulosa cells.

In the uterus, DRAGON protein was expressed in luminal and glandularepithelial cells of the endometrium (FIG. 27C, a and b). Weak stainingwas also found in circular muscle (FIG. 27C, a). Localization of DRAGONin the luminal and glandular epithelial cells indicate that DRAGON mayalso be required for normal endometrial function.

Cellular Localization of DRAGON in the Pituitary

Sporadic staining was observed in both the anterior and posterior lobesof the pituitary, whereas no staining was detected in the intermediatelobe (FIG. 28A). BMPs and their receptors are expressed in mousepituitary gonadotropes and in the LβT2 gonadotrope cell line(Paez-Pereda et al., Proc Natl Acad Sci USA 100:1034-1039, 2003; Otsukaet al., J Biol Chem 276:11387-11392, 2001), and BMPs can stimulate FSHbiosynthesis (Huang et al., Endocrinology 142:2275-2283, 2001). Toexamine whether FSH-expressing gonadotropes also express DRAGON, frozenpituitary sections were dual labeled with DRAGON and FSH antibodies.FSH-expressing cells indeed overlapped extensively, albeit notcompletely, with DRAGON-expressing cells (FIG. 28B). Interestingly, LβT2cells also express DRAGON (FIG. 29A). DRAGON may therefore influenceBMP-mediated FSH biosynthesis in vivo and in vitro.

DRAGON Expression in Cell Lines of the Reproductive Axis

Screening cell lines originating from reproductive organs (FIG. 29A)indicated that DRAGON was expressed in Hela (cervical carcinoma), MCF-7(breast carcinoma), LβT2 (pituitary carcinoma), JEG3 (placentacarcinoma), and Ishikawa (endometrium adenocarcinoma) cells. Incontrast, DRAGON mRNA was undetectable in S4 spermatogonial cells (Fenget al., Science 297:392-395, 2002) or KGN granulosa tumor cells (Nishiet al., Endocrinology 142:437-445, 2001).

Lipid Raft Localization of DRAGON

To explore the location of DRAGON on the cell surface using Ishikawacells, live cells were incubated with DRAGON antibody at 4° C., fixed,and processed for immunocytochemistry. DRAGON has a punctate pattern onthe cell membrane, typical of lipid raft proteins (FIG. 29B). DRAGONlocalization within membrane subdomains was then characterized byextracting cells on ice in the presence of 1% Triton X-100 and thensubjecting the cells to sucrose gradient ultracentrifugation. Asexpected, DRAGON was detected primarily within the low-density fractions(FIG. 29C; fractions 2 and 3), along with caveolin-1, which is typicallyassociated with lipid rafts. The high-density fractions, which includecellular and cytoskeletal proteins (fractions 6-9), contained β-actin,some caveolin-1, and a small amount of DRAGON. Thus, DRAGON is locatedwithin lipid rafts in Ishikawa cells.

DRAGON Enhances Signaling of BMP2 and BMP4

DRAGON is expressed in gonadal germ cells and in reproductive tractepithelial cells. Moreover, we have shown that DRAGON enhances the BMP2response in the HepG2 liver cell and LLC-PK1 kidney cell lines (Samad etal., J Biol Chem 280:14122-14129, 2005). To examine whether DRAGON has asimilar role in reproductive cells, Ishikawa and KGN cells weretransfected with DRAGON cDNA together with BRE-Luc, a BMP-responsiveluciferase reporter construct. DRAGON dose-dependently increased BREluciferase activity in both Ishikawa and KGN cells in the absence ofadded BMPs (FIG. 30A). DRAGON induces similar reporter activity in bothcell lines at lower doses (e.g., 0.1-10 ng), and a greater effect wasseen in Ishikawa cells at higher doses. Although DRAGON protein wasundetectable in Ishikawa cells before transfection, DRAGON wasdetectable after transfection with 100 ng cDNA (FIG. 30A, inset),indicating that the effects of transfected DRAGON are mediated by anincrease in DRAGON protein. Treatment of Ishikawa cells withnoggin-containing conditioned medium (100 ng/ml) resulted in partialinhibition of DRAGON-dependent BRE-Luc activity and completely inhibitedsignaling by BMP2 (10 ng/ml) (FIG. 30B). Conditioned medium frommock-transfected HEK293 cells had no inhibitory activity (FIG. 30C),indicating that the BMP-inhibiting activity in the noggin-conditionedmedium was caused by noggin itself, also indicating that the effect ofDRAGON on BMP reporter activity is dependent on endogenous ligand. Theeffect of DRAGON on BMP signaling in the presence of added BMP ligandswas examined next. Ishikawa cells were transfected with DRAGON cDNA andtreated with increasing doses of BMP ligands. DRAGON significantlyincreased BMP2 signaling at 11-700 pm BMP2 doses (P<0.05), but had noeffect at 2800 pm (FIG. 30D). Thus, at lower BMP2 doses (i.e., 11 or 44pm), DRAGON transfection resulted in detectable reporter activity thatis not seen in the absence of DRAGON (FIG. 30D). Similar results wereobserved with BMP4 and in KGN cells (data not shown). DRAGON thusincreases sensitivity of BMP-responsive cells to low concentrations ofendogenous or exogenous BMP ligands.

DRAGON is also expressed in many specific cell types throughout thereproductive system. DRAGON is also expressed in numerous cell linesderived from reproductive tissues, and DRAGON expression enhancesresponsiveness of Ishikawa and KGN cells to BMP2 and BMP4. DRAGONtherefore has important roles in mediating BMP signaling inreproduction. To define specific sites where DRAGON-mediated BMPsignaling is important in reproduction, cell-specific expression in themale and female reproductive tracts were explored. In males, BMP8a andBMP8b are expressed in maturing spermatocytes, and BMP8b knockout malesare infertile because of developmental arrest and degeneration ofspermatocytes (Zhao et al., Genes Dev 10:1657-1669, 1996; Zhao et al.,Development 125:1103-1112, 1998), suggesting that these BMPs arecritical for normal spermatocyte development. BMP receptors ALK3 andBMPR-II are localized in postnatal spermatogonia, and BMP4 is producedby Sertoli cells very early in postnatal development, consistent with anongoing requirement for BMP signaling in the testis (Pellegrini et al.,J Cell Sci 116:3363-3372, 2003). In addition, BMP2 primarily stimulatesspermatogonial proliferation, whereas BMP7 acts mainly on Sertoli cellsin the testis from 7d-old mice (Puglisi et al., Eur J Endocrinol151:511-520, 2004). Our results extend these findings to a novel BMPcoreceptor that enhances BMP signaling, because in 3d-old male mice,DRAGON was highly expressed in gonocytes before and after they migratedfrom the tubule lumen to their basal position, with thisimmunoreactivity being maintained as spermatogonia in d9 animals. Byd21, staining in spermatogonia was substantially diminished, butgonocytes remaining within the tubule lumen were still positive. Inadults, DRAGON staining appeared in maturing spermatocytes but not inother testicular cell types. The shift in expression from gonocytes andspermatogonia in juvenile animals to spermatocytes in mature malessuggests that the role of BMPs may change as the testes mature toproduce active sperm. Taken together, these results indicate a criticalrole for BMPs in regulating testis development and spermatogenesis andindicate that DRAGON is an important mediator of these processes.

BMP4, BMP7, and BMP8A are expressed in the epididymis, and knockout ofeach gene by itself resulted in degeneration of the epididymalepithelium (Hu et al., Dev Biol 276:158-171, 2004; Zhao et al., GenesDev 10:1657-1669, 1996; Zhao et al., Development 125:1103-1112, 1998),indicating that BMPs are involved in epididymal function. Interestingly,DRAGON was strongly expressed on the apical surface of polarizedepididymal epithelium in immature and mature males consistent with arole for DRAGON in enhancing this essential BMP signaling.

In females, both BMPs and their receptors have been identified innumerous ovarian cell types, including oocytes and granulosa cells(Shimasaki et al., Endocr Rev 25:72-101, 2004). In vitro studies havedemonstrated that BMP2, 4, 6, 7, and 15 regulate granulosa cellfunctions, and BMP4 and 7 promote the primordial-to-primary follicletransition during follicle maturation (reviewed in Shimasaki et al.,Endocr Rev 25:72-101, 2004). The significance of BMP signaling inovarian function is also underscored by the altered ovulation rates inInverdale sheep with a natural point mutation in the BMP15 gene(Galloway et al., Nat Genet 25:279-283, 2000) and in Booroola sheep witha point mutation in the BMPRIB gene (Fabre et al., J Endocrinol177:435-444, 2003). In the ovary, DRAGON is expressed exclusively inoocytes and most prominently in oocytes within secondary follicles. Thisis a time of oocyte growth and cytoplasmic maturation (Eppig et al.,Reprod Fertil Dev 8:485-489, 1996), suggesting that BMP signaling ingeneral, and DRAGON enhancement of this signaling in particular, isimportant for growth and maturation of oocytes.

BMP signaling components are expressed in a variety of cells within therat uterus (Erickson et al., J Endocrinol 182:203-217, 2004). BMP2 mRNAis restricted to periluminal stroma, and BMP7 is expressed inperiluminal stroma and glandular epithelial cells, whereas BMP4 and BMP6are expressed in blood vessels in the uterus. BMPRIA, BMPRIB, and BMPRIIare expressed in a number of cell types in the uterus including luminaland glandular epithelial cells. DRAGON is expressed in luminal andglandular epithelial cells of the mouse endometrium, suggesting thatDRAGON may enhance BMP signals involved in regulating uterine maturationin preparation for implantation.

BMP2, 4, 6, 7, and 15 are expressed in mouse pituitary, and BMP6, 7, and15 have been shown to stimulate FSH synthesis and secretion (Paez-Peredaet al., Proc Natl Acad Sci USA 100:1034-1039, 2003; Huang et al.,Endocrinology 142:2275-2283, 2001; Otsuka et al., Endocrinology143:4938-4941, 2002). BMP6 and BMP7 can also stimulate FSH mRNAbiosynthesis in LβT2 mouse pituitary cells in culture (Huang et al.,Endocrinology 142:2275-2283, 2001). DRAGON expression in LβT2 cells aswell as in numerous cells within the mouse pituitary, some of which alsostained for FSH, can be observed. BMPs may therefore act in an autocrinemanner to modulate FSH biosynthesis and DRAGON may enhance this process.

In cell culture studies using cell lines from the reproductive tract,DRAGON expression enhanced the response to endogenous BMP ligand as wellas low doses of exogenous BMP2 and BMP4, results that agree with ourobservations in nonreproductive cell lines (Samad et al., J Biol Chem280:14122-14129, 2005). Immunocytochemical analysis indicates thatDRAGON is located on the plasma membrane in discrete patches, consistentwith its belonging to the class of GPI-anchored proteins that are knownto localize in lipid rafts (Fullekrug et al., Ann NY Acad Sci1014:164-169, 2004). Moreover, our work indicates that DRAGON caninteract directly with BMPRII, ActRII, and the Alk3 and Alk6 type Ireceptors (Samad et al., J Biol Chem 280:14122-14129, 2005). Takentogether, these results provide a model for enhanced BMP signaling inwhich DRAGON acts as a BMP coreceptor in collecting type II and type Ireceptors into lipid rafts where they are optimized to respond to lowdoses of BMP ligands. Because DRAGON can bind BMP2 and BMP4 directly,DRAGON may act to stabilize the ligand-receptor complex in lipid rafts,thereby facilitating endocytosis and signaling. Of course, these twopossibilities are not mutually exclusive. Based on the localizedexpression of DRAGON in developing and maturing germ cells, as well asspecific epithelial cells within the reproductive tract that are knownto be BMP responsive, BMPs may therefore play an important role inregulating reproduction in mammals and that this role may be regulatedby DRAGON.

Experiments related to DRAGON's role in reproduction were carried out asfollows.

Animals

Mice [B6C3F1 (C57BI/6×C³H)] were maintained in the animal barrierfacility and killed at different ages to collect tissues forimmunohistochemistry and RNA extraction. In addition, mice at 19 d ofage were injected with pregnant mare serum gonadotropin (PMSG; ip, 5IU/mouse; Sigma Chemical Co., St. Louis, Mo.), and killed 48 h later tocollect gonads for immunohistochemistry.

RT-PCR

Total RNA was extracted from tissues stored in RNAlater (Ambion, Austin,Tex.) or cells stored in Trizol (Life Technologies, Inc., Carlsbad,Calif.) according to the manufacturer's protocol. Total RNA (0.5-1.0 μg)was reverse transcribed as previously described (Sidis et al., BiolReprod 59:807-812, 1998). Aliquots (2 μl) of first-strand cDNA mix wereused in PCR (35 cycles) to amplify DRAGON and β-actin. The primers,which amplify both human and mouse DRAGON cDNA, were TGT TCC AAG GAT GGACCC ACA TC (forward; SEQ ID NO:14) and GCA GGT CAT CTG TCA CAG CTT GG(reverse; SEQ ID NO:15).

Immunohistochemistry and Immunocytochemistry

A rabbit polyclonal antibody was raised against a peptide correspondingto the C terminus of DRAGON upstream of its GPI anchor. This antibodyhas been shown to specifically recognize DRAGON protein (Samad et al., JNeurosci 24:2027-2036 2004).

Immunohistochemistry on paraffin sections were performed as previouslydescribed (Xia et al., Mol Endocrinol 18:979-994, 2004). Briefly,ovaries were fixed in Bouin's solution, and other organs were fixed in4% paraformaldehyde. Antigen retrieval was performed on paraffinsections in 0.01 m citrate buffer (pH 6.0). Tissue sections wereincubated overnight with anti-DRAGON (1:4000), washed, incubated for 1 hwith biotinylated goat antirabbit IgG and then 30 min with VectastainElite ABC (Vector Laboratories, Inc., Burlingame, Calif.), and developedwith diaminobenzidine (DAB) for detection (ICN Biomedical, Inc., Aurora,Ohio). Sections were then counterstained with Harris' hematoxylin.

To colocalize DRAGON and FSH in the pituitary, mouse pituitaries werefixed in 4% paraformaldehyde at 4° C. overnight, cryoprotected in 30%sucrose overnight, and frozen in Tissue-Tek OCT embedding compound(Electron Microscopy Sciences, Fort Washington, Pa.). Sections (12 μm)were incubated with a mixture of rabbit anti-DRAGON serum (1:2000) andguinea pig antimouse FSH (1:1600, AFP-3080, National Institute ofDiabetes and Digestive and Kidney Diseases, Bethesda, Md.) for 1 h,washed, and then with a mixture of fluorescein isothiocyanate(FITC)-conjugated donkey antirabbit IgG and tetramethyl rhodamineisothio-cyanate-conjugated donkey anti-guinea pig IgG (diluted 1:200;Jackson ImmunoResearch Laboratories, Inc., West Grove, Pa.).

For immunocytochemistry, Ishikawa cells were grown on glass coverslips.Live cells were incubated with anti-DRAGON serum (1:2000) for 1 h onice. Cells were then fixed in 2% paraformaldehyde for 20 min. The boundantibodies were detected by incubating with FITC-conjugated donkeyantirabbit IgG (diluted 1:200 in PBS) for 1 h. To demonstratespecificity, the DRAGON antibody was preincubated overnight with 10 mmimmunization peptide before being applied to sections or cells.

In Situ Hybridization

Air-dried frozen sections (14-18 μm) were fixed in 4%paraformal-dehyde-PBS, digested with proteinase K, acetylated, washed,and dehydrated. Antisense and sense cRNA probes were prepared by meansof in vitro transcription in the presence of [α-³⁵S]UTP, which were thenhybridized in 50% deionized formamide, 10 mm Tris/HCl (pH 7.6), 600 mmNaCl, 0.25% SDS, 200 μg/ml yeast tRNA, 50 mm dithiothreitol, 1Denhardt'ssolution, and 10% dextran sulfate overnight at 55° C. in a humidifiedchamber. After hybridization, the sections were incubated withribonuclease A and washed in 0.1SSC containing 13-mercapto-ethanol (875μl in 300 ml) and 0.5 mm EDTA at 65° C. for 1 h. After developing, theslides were counterstained with hematoxylin and mounted for photography.

Lipid Raft Protein Extraction

Lipid raft proteins were prepared after protocols previously described(20, 21). Briefly, Ishikawa cells were scraped and pelleted in ice-oldPBS, resuspended in 2 ml ice-cold lysis buffer [10 mm Tris/HCl (pH 7.5),150 mm NaCl, 1% Triton X-100, 2 mm EDTA, and proteinase inhibitors], andallowed to stand on ice for 30 min. The lysate was centrifuged for 5 minat 1300 g to remove nuclei and large cellular debris. The supernatantwas mixed with an equal volume of 85% sucrose in TBS [10 mm Tris/HCl (pH7.5), 150 mm NaCl], placed at the bottom of a 10-ml ultracentrifugetube, and then overlaid with 5 ml of 35% sucrose and 1.4 ml of 5%sucrose. The sample was then centrifuged for 14 h at 150,000 g in aSW41Ti rotor so that rafts could float to the tip while cytoskeletal andcytoplasmic proteins remain at the bottom. Five fractions of 1 ml andfour fractions of 1.35 ml were collected from the top of the tube. Thelight-scattering band, an indicator of the location of lipid rafts(Mukherjee et al., J Biol Chem 278:40806-40814, 2003), was locatedprimarily in fraction 2. These fractions were analyzed bytrichloroacetic acid precipitation of proteins from 100-0 aliquotsfollowed by SDS-PAGE and Western blotting.

Western Blotting

Western blotting analyses were performed as previously described (Xia etal., Mol Endocrinol 18:979-994, 2004). Briefly, samples from sucrosegradient fractions were subjected to SDS-PAGE under reducing conditions,transferred to a polyvinylidene difluoride membrane (Millipore, Bedford,Mass.), blocked in 10% nonfat dry milk, and incubated overnight at 4° C.with rabbit anti-DRAGON (1:4000) or anti-caveolin-1 (1:2000; BDBiosciences, San Jose, Calif.) antibodies. The membranes were washedthree times before being incubated for 2hat room temperature with secondantibody, which was detected with enhanced chemiluminescence (ECL)Reagent Plus (PerkinElmer Life Sciences, Boston, Mass.). After exposure,membranes were stripped for 30 min at 50° C. and reprobed with amonoclonal β-actin antibody (1:1000; Santa Cruz Biotechnology, SantaCruz, Calif.).

Transfection and Luciferase Assay

Ishikawa and KGN cells were maintained in TT medium [1:1 mixture of DMEMand F-12, supplemented with 1% 1-glutamine, 100 IU/ml penicillin, 100μg/ml streptomycin sulfate, and 10% fetal bovine serum (LifeTechnologies, Inc., Rockville, Md.)]. To examine the effect of DRAGON onBMP signaling, transfections were performed in 24-well trays usingLipofectamine 2000 (Invitrogen, Carlsbad, Calif.) with a total of 400 ngDNA [180 ng BRE-Luc, a BMP response element kindly provided by Dr. tenDijke (Korchynskyi et al., J Biol Chem 277:4883-4891, 2002), 10 ngpRL-TK, and the indicated doses of DRAGON cDNA and pcDNA3].Approximately 24 h after transfection, the medium was replaced withserum-free TT medium supplemented with 0.1% BSA, with or without BMPligands (R&D Systems, Minneapolis, Minn.). After treating for 16 h, thecells were lysed and assayed for luciferase activity using the dualluciferase reporter assay kit (Promega, Madison, Wis.).

To obtain noggin protein for neutralization of endogenous BMPs, thehuman noggin cDNA (IMAGE clone 4737725 from American Type CultureCollection, Rockville, Md.) was subcloned into pcDNA3 (Invitrogen) andtransfected into HEK-293-F suspension cultures in Freestyle serum-freemedium (Invitrogen) as previously described (Keutmann et al., MolEndocrinol 18:228-240, 2004). Concentrated conditioned medium wascalibrated by two independent methods including 1) biological assay and2) Western blotting. In the biological assay, increasing amounts ofnoggin-conditioned medium were mixed with 10 ng/ml BMP2 and used totreat HepG2 cells that were previously transfected with the BRE-Lucreporter. At the effective concentrations for half-maximal response(EC50), the amount of noggin in the culture medium was equal to that ofthe BMP2 concentration, assuming molar equivalent antagonism activity(Zimmerman et al., The Spemann Cell 86:599-606, 1996). For the Westernblotting analysis, serial dilutions of noggin-conditioned medium wereresolved by 12% PAGE under reducing conditions and visualized bystaining with an antimouse noggin polyclonal antibody (R&D Systems).Noggin concentrations were estimated at the detection limit doseaccording to the manufacturer's information. Both methods gave similarresults. As a control, concentrated conditioned medium frommock-transfected HEK293 cells was tested and determined to have noBMP-inhibitory activity.

To demonstrate that Ishikawa cells transfected with DRAGON cDNA actuallyproduce DRAGON protein, transfected cells were extracted in RIPA buffer[150 mm NaCl, 50 mm Tris (pH 7.5), 1 mm EDTA, 50 mm NaF, 0.5% NonidetP-40, 0.5% deoxycholic acid, and 0.1% SDS], and the lysates were thenanalyzed by Western blotting for DRAGON as described above.

Data Analysis

FIGS. 30A, 30C, and 30D depict the mean±SE of triplicates fromrepresentative experiments. In vitro bioassay experiments in thepresence or absence of transfected DRAGON (FIG. 30D) represents the meanSE of six determinations from three independent experiments and wereanalyzed by two-way ANOVA. Differences between ligand doses or betweenpresence and absence of DRAGON were identified by Student-Newman-Keulspost hoc test. Differences of P<0.05 were considered significant.

DRAGON Homolog RGMa

A DRAGON homolog, RGMa, has similar functionality to DRAGON, and mayalso be used in the methods of the invention.

Regulation of the signal transduction pathway occurs at many levels. Onekey regulatory mechanism for many TGF-β superfamily members is throughaccessory or co-receptors to promote or inhibit ligand binding (Shi andMassague, Cell 113:685-700, 2003; Lopez-Casillas et al., Cell73:1435-1444, 1993; Shen and Schier, Trends Genet. 16:303-309, 2000;Cheng et al., Genes Dev. 17:31-36, 2003; Gray et al., Proc. Natl. Acad.Sci. USA 100:5193-5198, 2003; Samad et al., J. Biol. Chem.280:14122-14129, 2005). For example, the TGF-β type III receptor(betaglycan) mediates binding of TGF-132 to the type II receptor and isimportant for TGF-β2 signaling (Lopez-Casillas et al., Cell73:1435-1444, 1993). Glycosylphosphatidylinositol (GPI)-linked proteinsfrom the epidermal growth factor-Cripto-Criptic-FRL-1 family areco-receptors necessary for nodal, Vg1, and growth and differentiationfactor 1 signaling (Shen and Schier, Trends Genet. 16:303-309, 2000;Cheng et al., Genes Dev. 17:31-36, 2003). Cripto also inhibits activinsignaling by preventing binding of the activin/type II receptor complexto type I receptors (Gray et al., Proc. Natl. Acad. Sci. USA100:5193-5198, 2003). We have recently identified the GPI-anchoredprotein DRAGON (RGMb) as the first co-receptor for BMP signaling (Samadet al., J. Biol. Chem. 280:14122-14129, 2005). DRAGON enhances cellularresponses to BMP, but not TGF-β, signals in a ligand-dependent manner.DRAGON associates with BMP type I and type II receptors, and solubleDRAGON.Fc fusion protein binds selectively to BMP-2 and BMP-4, but notBMP-7 or other members of the TGF-β superfamily of ligands (Samad etal., J. Biol. Chem. 280:14122-14129, 2005).

DRAGON is a member of the repulsive guidance molecule (RGM) family ofgenes, which also includes RGMa and hemojuvelin (HJV/RGMc/HFE2). Thesefamily members share 50-60% amino acid identity and similar structuralfeatures, including an N-terminal signal sequence, conserved proteolyticcleavage site, partial von Willebrand factor type D domain, and GPIanchor (Monnier et al., Nature 419:392-395, 2002.; Papanikolaou et al.,Nat. Genet. 36:77-82, 2004; Samad et al., J. Neurosci. 24:2027-2036,2004; Niederkofler et al., J. Neurosci. 24:808-818, 2004; Schmidtmer andEngelkamp, Gene Expr. Patterns 4:105-110, 2004; Oldekamp et al., GeneExpr Patterns 4:283-288, 2004). Unlike DRAGON, RGMa and hemojuvelin alsopossess an RGD motif, which could be involved in cell attachment(Monnier et al., Nature 419:392-395, 2002). RGMa and DRAGON areexpressed in a complementary manner in the central nervous system (Samadet al., J. Neurosci. 24:2027-2036, 2004; Niederkofler et al., J.Neurosci. 24:808-818, 2004; Schmidtmer and Engelkamp, Gene Expr.Patterns 4:105-110, 2004; Oldekamp et al., Gene Expr Patterns 4:283-288,2004), where RGMa mediates repulsive axonal guidance (Monnier et al.,Nature 419:392-395, 2002; Rajagopalan et al., Nat. Cell Biol. 6:756-762,2004; Brinks et al., J. Neurosci. 24:3862-3869, 2004), and neural tubeclosure (Niederkofler et al., J. Neurosci. 24:808-818, 2004), whileDRAGON contributes to neuronal cell adhesion through homophilicinteractions (Samad et al., J. Neurosci. 24:2027-2036, 2004). RGMa alsobinds to the receptor neogenin (Rajagopalan et al., Nat. Cell Biol.6:756-762, 2004) and functions as a cell survival factor (Matsunaga etal., Nat. Cell Biol. 6:749-755, 2004). Hemojuvelin is expressed mostheavily in the liver, heart, and skeletal muscle, and is mutated injuvenile hemochromatosis, a disorder of iron overload (Papanikolaou etal., Nat. Genet. 36:77-82, 2004; Samad et al., J. Neurosci.24:2027-2036, 2004; Niederkofler et al., J. Neurosci. 24:808-818, 2004;Schmidtmer and Engelkamp Gene Expr. Patterns 4:105-110, 2004; Oldekampet al., Gene Expr. Patterns 4:283-288, 2004; Rodriguez Martinez et al.,Haematologica 89:1441-1445, 2004).

RGMa is involved in the BMP signaling pathway. A reporter assay showsthat transfection of RGMa cDNA into cells enhances BMP, but not TGF-β,signals in a ligand-dependent fashion. Binding and crosslinking studiesin a cell-free system demonstrate that soluble RGMa.Fc fusion proteininteracts with the BMP type I receptor ALK6 and binds directly to¹²⁵I-BMP-2 and ¹²⁵I-BMP-4, but not other members of the TGF-βsuperfamily. Co-transfection of RGMa cDNA with dominant negative BMPtype I receptors or with dominant negative Smad1 inhibits RGMa-mediatedBMP signaling, suggesting that RGMa generates BMP signals via theclassical BMP pathway. Transfection of RGMa cDNA into cells inducesphosphotylation of endogenous Smad1/5/8 and upregulates endogenous Id1.Finally, immunofluorescence microscopy of adult rat spinal cordsections, reveals that RGMa is expressed in vivo in neurons which alsoshow nuclear accumulation of p-Smad1/5/8. Taken together, these dataindicate that RGMa functions as a BMP co-receptor.

RGMa Mediates BMP, but not TGF-β, Signaling

As RGMa homolog DRAGON functions as a BMP co-receptor in LLC-PK1 porcinekidney epithelial cells (Samad et al J. Biol. Chem. 280:14122-14129,2005), the ability of RGMa to mediate BMP signaling was tested in thesecells. LLC-PK1 cells were transfected with a BMP-responsive luciferasereporter (BRE-Luc, Korchynskyi and ten Dijke J. Biol. Chem277:4883-4891, 2002) (FIGS. 31A and 31C) or a TGF-β responsiveluciferase reporter (CAGA-Luc, Dennler et al., EMBO J. 17:3091-3100,1998) (FIG. 31B) either alone or in combination with cDNA encoding RGMa.Transfected cells were then incubated with or without BMP-2 or TGF-β1for 16 hours followed by measurement of luciferase activity. In theabsence of RGMa, stimulation with BMP-2 or TGF-β1 increased the relativeluciferase activity for their respective reporters compared withunstimulated cells (FIGS. 31A and 31B, compare bars 2 to 1).Co-transfection with RGMa similarly increased BRE luciferase activityeven in the absence of exogenous BMP ligand (FIG. 31A, bar 3). TheRGMa-mediated BMP signaling was dose dependent (FIG. 31C, white bars),reaching a peak at about 200 ng cDNA per transfection. RGMa alsoaugmented signaling produced by exogenous BMP-2 (FIG. 31C, black bars).In contrast, co-transfection with RGMa (up to 1 m g) did not increasethe TGF-β responsive CAGA luciferase activity above baseline (FIG. 31B,bar 3). Similar results. were seen in another cell line (HepG2 cells,data not shown). Taken together, these results demonstrate that likeDRAGON, RGMa mediates BMP, but not TGF-β, signaling.

RGMa-Mediated BMP Signaling is Ligand-Dependent

The ability of RGMa to generate BMP signals even in the absence ofexogenous BMP ligand raises the question of whether RGMa acts in aligand-independent manner, or whether it augments signaling byendogenous BMP ligands. To investigate this question, we examinedwhether RGMa-mediated signaling was inhibited by noggin, a soluble BMPinhibitor that binds and sequesters BMP ligands barring access tomembrane receptors (Balemans and Van Hul Dev. Biol. 250:231-250, 2002;Groppe et al., Nature 420:636-642, 2002). The effects of noggin onexogenous BMP-2 and TGF-β1 stimulation were used as positive andnegative controls respectively. Results were confirmed using aneutralizing antibody against BMP-2 and BMP-4 (α-BMP-2/4). In theabsence of noggin, co-transfection with RGMa cDNA increased BREluciferase activity 8-fold above baseline (FIG. 32A, compare bar 3 to1). Similarly, exogenous BMP-2 increased BRE luciferase activity 15-foldover baseline (FIG. 32A, compare bar 5 to 1). This stimulation by eitherRGMa transfection or exogenous BMP-2 was blocked by noggin (FIG. 32Abars 4, 6). Noggin also decreased basal BMP signaling in cells neithertransfected with RGMA nor stimulated with exogenous BMP-2 (FIG. 32A,compare bar 2 to 1). In contrast, noggin did not affect TGF-β1 inducedCAGA luciferase activity (data not shown). Similar results were seenwith a neutralizing antibody against BMP-2 and BMP-4 (FIG. 32B). Thus,RGMa generates BMP signals in a ligand-dependent manner, likely viaendogenously expressed BMP ligands. The observation of mRNA for bothBMP-2 and BMP-4 in these cells by RT-PCR further supports thispossibility (FIG. 32C).

Production and Characterization of Soluble RGMa.Fc Fusion Protein

An RGMa.Fc fusion protein was produced by fusing the extracellulardomain of RGMa to the Fc portion of human IgG. An affinity purifiedrabbit polyclonal antibody raised against a C-terminal peptide sequenceof RGMa upstream of its GPI anchor (α-RGMa) was also generated. PurifiedRGMa.Fc was analyzed by reducing SDS-PAGE followed by Western blot usinganti-human Fc antibody (α-Fc) and α-RGMa. Both antibodies recognized twobands of approximately 60 and 75 kDa not seen in mock transfected cells,confirming the presence of both the RGMa and Fc domains, and validatingthe RGMa antibody (FIG. 33). These bands were not seen when the RGMaantibody was pre-incubated with competing antigenic peptide (data notshown). The larger band is consistent with the predicted size of thefull length RGMa.Fc fusion protein, and the smaller band is consistentwith the predicted size of RGMa.Fc fusion protein which has beenproteolytically cleaved as described for both the chick in Monnier etal. (Nature 419:392-395, 2002) and mouse homologues in Niederkofler etal. (J. Neurosci. 24:808-818, 2004) of RGMa.

RGMa.Fc Binds Selectively to BMP-2 and BMP-4, but not BMP-7 or TGF-β1

Next, RGMa was tested for direct interactions with BMP ligands usingsoluble RGMa.Fc fusion protein in a cell free binding system. RGMa.Fcwas incubated overnight with ¹²⁵I-BMP-2 with or without excess coldBMP-2, -4, -7 or TGF-β1, followed by incubation on protein A coatedplates and determination of radioactivity. RGMa.Fc bound to ¹²⁵I-BMP-2(FIG. 34A, compare bar 2 to 1). This binding was competitively inhibitedby excess cold BMP-2 or BMP-4, but not by BMP-7 or TGF-β1. Similarfindings were seen with ¹²⁵I-BMP-4 (data not shown).

Chemical crosslinking experiments using DSS in a cell free systemprovided additional support for interaction between RGMa.Fc and BMP-2.¹²⁵I-BMP-4 was crosslinked with RGMa.Fc in the presence of DSS (FIG.34B, bar 4), and this crosslinking was inhibited by excess cold BMP-4(FIG. 34B, bar 5). No band was seen in the absence of DSS (FIG. 34B,bars 1-2) or when buffer alone (FIG. 34B, bar 3) or ALK5.Fc (a TGF-βtype I receptor, FIG. 34B, bar 6) was used in place of RGMa.Fc. Similarresults were seen for crosslinking with ¹²⁵I-BMP-2 (data not shown).Taken together, these data indicate that RGMa.Fc binds directly andselectively to BMP-2 and BMP4, but not BMP-7 or TGF-β1.

RGMa Mediates BMP Signaling Via BMP Type I Receptors

Determination of whether RGMa acts via the classical BMP signalingpathway through BMP receptors was made. Dominant negative mutants of BMPtype I receptors ALK3 (ALK3 DN) and ALK6 (ALK6 DN), which are deficientin kinase activity and therefore unable to phosphorylate Smad1/5/8, havebeen described in Clarke et al. (Mol. Endocrinol. 15:946-959, 2001) andChen et al. (J. Cell Biol. 142:295-305), 1998). The effects ofco-transfection with dominant negative ALK3 and ALK6 mutants onRGMa-mediated BMP signaling were examined, and the effect of thesemutants on exogenous BMP was used as a control. Transfection with RGMaor incubation of cells with exogenous BMP-2 increased BRE luciferaseactivity 6-10-fold above baseline (FIG. 35A, compare bar 2 to 1 and 6 to5). This stimulation by either RGMa or exogenous BMP-2 was blocked byco-transfection with dominant negative ALK3 (FIG. 35A, bars 3, 7) ordominant negative ALK6 (FIG. 35A, bars 4, 8).

To determine whether RGMa interacted directly with BMP type I receptors,purified RGMa.Fc, ALK6.Fc, and/or BMP-2 were incubated in solutioneither individually or in various combinations, in the presence of thecrosslinker DSS. Complexes were pulled down with protein A beads andanalyzed by non-reducing SDS-PAGE, followed by Western blot with α-RGMa.RGMa.Fc formed a complex in solution with BMP-2, demonstrated by a moreslowly migrating band under non-reducing conditions compared withRGMa.Fc alone (FIG. 35B, compare arrowhead in lane 4 to lane 3). RGMa.Fcalso formed a complex with ALK6.Fc, even in the absence of BMP-2 (FIG.35B, compare arrowhead in lane 5 to lane 3). In the presence of BMP-2,an even larger shift was seen, indicating that a complex containingRGMa.Fc, ALK6.Fc, and BMP-2 had formed (FIG. 35B, lane 6, arrowhead). Nobands were seen for ALK6.Fc or BMP-2 in the absence of RGMa.Fc (FIG.35B, lanes 1 and 2). BMP ligands exhibit high affinity for BMP type Ireceptors and low affinity for BMP type II receptors (Shi and Massague,Cell 113:685-700, 2003). The combination of RGMa and BMP type Ireceptors increased binding of BMP ligands compared with BMP type Ireceptors alone was thus tested. Purified RGMa.Fc and ALK6.Fc wereincubated overnight in solution with ¹²⁵I-BMP-2, followed by incubationon protein A coated plates and determination of radioactivity. RGMa.Fcand ¹²⁵I -ALK6.Fc alone each significantly bound BMP-2 (FIG. 35C,compare bars 2 and 3 to 1). As a negative control, BMP type II receptorwas unable to bind (data not shown). The combination of RGMa.Fc andALK6.Fc increased binding to ¹²⁵I-BMP-2 compared with ALK6.Fc alone(FIG. 35C, compare bar 4 to 3).

RGMa Mediates BMP Signaling Via Smad1/5/8, and Upregulates EndogenousId1 Expression

The role of RGMa in the classical BMP signaling pathway was studiedusing the effects of wildtype Smad1 (WT Smad1) versus dominant negativeSmad1 (DN Smad1), a dominant negative mutant of Smad1 with deletedcarboxy terminal phosphoacceptor residues (Samad et al., J. Biol. Chem.280:14122-14129, 2005; Macias-Silva et al., J. Biol. Chem.273:25628-25636, 1998, Piscione et al., Am. J. Physiol. Renal Physiol.280:F19-33, 2001), on RGMa-mediated BMP signaling. Results were comparedwith their effects on exogenous BMP-2 signaling as a control. Consistentwith other studies (Samad et al., J. Biol. Chem. 280:14122-14129, 2005;Hoodless et al., Cell 85:489-500, 1996; Macias-Silva et al., Cell87:1215-1224, 1996), transfection with WT Smad1 alone increased BREluciferase activity 8-fold above baseline (FIG. 36A, compare bar 2 to1), while transfection with DN Smad1 alone decreased BRE luciferaseactivity below baseline (FIG. 36A, compare bar 3 to 1). Transfectionwith RGMa increased BRE luciferase activity 7-fold above baseline (FIG.36A, bar 4). Co-transfection of WT Smad1 with RGMa further augmented thesignaling induced by either WT Smad1 or RGMa alone (FIG. 36A, comparebar 5 to 2, 4). Co-transfection of DN Smad1 with RGMa blocked theincrease in signal seen with RGMa alone (FIG. 36A, compare bar 6 to 4).Similar results were seen for the effects of WT Smad1 and DN Smad1 onexogenous BMP-2 stimulation (FIG. 36A, bars 7-10).

To demonstrate that RGMa mediates BMP signaling through the Smadsignaling pathway, the effect of RGMa expression on phosphorylation ofendogenous Smad1/5/8 was studied. LLC-PK1 cells were transientlytransfected with RGMa cDNA and cell lysates were assayed for p-Smad1/5/8by Western blot (FIG. 36B, α-p-Smad1/5/8). Blots were stripped andreprobed for total Smad1 as a loading control (FIG. 36B, α-Smad1).Results were compared to mock transfected cells as a negative control,and cells stimulated for 2 hours with 50 ng/ml exogenous BMP-2 as apositive control. Expression of p-Smad1/5/8 relative to Smad1 wasquantitated using IPLab Spectrum software (FIG. 36C). Consistent withour other data supporting the notion of endogenous BMP signaling inthese cells, mock transfected cells (without RGMa or exogenous BMP-2stimulation) did have some basal level of p-Smad1/5/8 (FIG. 36B, lefttwo lanes). Transfection with RGMa cDNA increased p-Smad1/5/8 levelscompared with mock transfected cells (FIG. 36B, compare middle threelanes to left two lanes; FIG. 36C, compare bar 2 to 1). As a positivecontrol, stimulation with exogenous BMP-2 also increased p-Smad1/5/8levels (FIG. 36B, right two lanes; FIG. 36C, bar 3).

We then demonstrated that RGMa affects expression of endogenous Id1, animportant downstream target of BMP signals (Hollnagel et al., J. Biol.Chem. 274:19838-19845, 1999; Korchynskyi and ten Dijke J. Biol. Chem.277:4883-4891, 2002; Lopez-Rovira et al., J. Biol. Chem. 277:3176-3185,2002; Miyazono and Miyazawa, Sci STKE. 2002:PE40, 2002; ten Dijke etal., Mol. Cell. Endocrinol. 211:105-113, 2003). Western blots used inthe p-Smad1/5/8 assay above were stripped and re-probed with anti-Id1antibody (FIG. 36B, α-Id1). Blots were stripped again and re-probed withactin antibody (FIG. 36B, α-β-actin) as a loading control, and the ratioof Id1 to β-actin expression was quantitated using IPLab Spectrumsoftware (FIG. 36D). Transfection with RGMa increased expression of Id1protein about 2.3-fold compared with mock transfected cells (FIG. 36Bcompare middle three lanes to left two lanes; FIG. 36D compare bar 2 to1). As a positive control, stimulation with exogenous BMP-2 alsoincreased Id1 expression (FIG. 36B, right two lanes; FIG. 36D, bar 3).Thus, RGMa mediates BMP signaling via the classical BMP pathwayinvolving Smad1/5/8, and RGMa increases expression of endogenous Id1protein, a downstream target of BMP signals.

RGMa is Widely Expressed

Our studies are the first to examine expression of RGMa in a widevariety of tissues. Other studies have focused on detailing theexpression pattern of endogenous RGMa in the central nervous system andduring development (Samad et al., J. Neurosci. 24:2027-2036, 2004;Niederkofler et al., J. Neurosci. 24:808-818, 2004; Schmidtmer andEngelkamp Gene Expr. Patterns 4:105-110, 2004; Oldekamp et al., GeneExpr Patterns 4:283-288, 2004). To begin to elucidate the role of RGMain vivo, we performed Northern blot analysis of endogenous RGMaexpression in a variety of adult rat tissues. RGMa message is widelyexpressed in many of the tissues tested, including heart, brain, lung,liver, skin, kidney, and testis (FIG. 37). Two distinct bands were seenin some tissues, possibly representing alternative transcriptioninitiation or alternative splicing.

BMP Signaling Occurs in Neurons of the Adult Spinal Cord which ExpressRGMa

Next, we demonstrated that RGMa expression in vivo correlates with itshypothesized role as a co-receptor for BMP signaling. RGMa mRNA has beenis widely expressed in a complementary fashion to DRAGON in the centralnervous system (Samad et al., J. Neurosci. 24:2027-2036, 2004;Niederkofler et al., J. Neurosci. 24:808-818, 2004; Schmidtmer andEngelkamp Gene Expr. Patterns 4:105-110, 2004; Oldekamp et al., GeneExpr Patterns 4:283-288, 2004), including ventral horn neurons of thespinal cord (Samad et al., J. Neurosci. 24:2027-2036, 2004). Wetherefore determined whether RGMa protein was expressed in ventral hornneurons, and we examined whether these neurons also showed evidence ofBMP signaling, i.e., nuclear accumulation of p-Smad1/5/8. Adult ratspinal cord sections were analyzed by immunofluorescence microscopy withα-RGMa, α-p-Smad1/5/8, and/or anti-neuron-specific nuclear proteinantibody (α-NeuN) to visualize neuronal cell bodies (Samad et al., J.Neurosci. 24:2027-2036, 2004). RGMa staining colocalized with NeuNstaining in ventral horn motor neurons (FIGS. 38A-38C). Ventral hornmotor neurons were also positive for nuclear p-Smad1/5/8 (FIGS.38D-38F), suggesting that there is basal signal transduction via the BMPpathway in these cells. Thus, endogenous RGMa is expressed in ventralhorn motor neurons which also generate BMP signals, indicating a rolefor RGMa as a BMP co-receptor in vivo.

BMPs are members of the TGF-β superfamily of ligands which play apleitropic role in vertebrate development and adult tissues (Hogan,Genes Dev. 10:1580-1594, 1996; Zhao, Genesis 35:43-56, 2003; Balemansand Van Hul Dev. Biol. 250:231-250, 2002). These functions require tightspatiotemporal regulation and specific activation via receptor complexesof particular intracellular signaling pathways. In order to generatespecificity and to finely tune these signals, regulation occurs atmultiple levels extracellularly, at the membrane surface, andintracellularly (Shi and Massague, Cell 113:685-700, 2003; Balemans andVan Hul Dev. Biol. 250:231-250, 2002; von Bubnoff and Cho Dev. Biol.239:1-14, 2001).

For the BMP pathway, most regulatory mechanisms identified to date areinhibitory. Soluble BMP antagonists such as noggin, chordin,chordin-like, follistatin, FSRP, DAN, cerebrus, and gremlin bind BMPs inthe extracellular space and mask receptor binding interfaces for BMPtype I and type II receptors (Balemans and Van Hul Dev. Biol.250:231-250, 2002; Groppe et al., Nature 420:636-642, 2002). At themembrane surface, BAMBI (BMP and activin receptor membrane boundinhibitor), which is structurally related to type I receptors in theextracellular domain but lacks the intracellular serine/threonine kinasedomain, inhibits BMP signals by stably associating with type IIreceptors and preventing formation of the active receptor complexes(Onichtchouk et al., Nature 401:480-485, 1999). Inhibin, in concert withits co-receptor betaglycan (also known as the TGF-β type III receptor),competes with BMPs for access to BMP type II receptors (Wiater and Vale,J. Biol. Chem. 278:7934-7941, 2003). Inside the cell, inhibitory Smads(Smad 6 and Smad 7) inhibit signaling by either interacting withphosphorylated type I receptors to prevent activation ofreceptor-activated Smads (Imamura et al., Nature 389:622-626, 1997;Nakao et al., Nature 389:631-635, 1997; Souchelnytskyi et al., J. Biol.Chem. 273:25364-25370, 1998), or through competition to preventformation of the receptor-activated Smad/Co-Smad complex (Hata et al.,Genes Dev. 12:186-197, 1998). Other intracellular molecules Smurf1 andSmurf2 (Smad ubiquitination regulatory factors), selectively targetactivated type I receptors and Smad proteins for degradation (Zhu etal., Nature 400:687-693, 1999; Kaysak et al., Mol. Cell 6:1365-1375,2000; Zhang et al., Proc. Natl. Acad. Sci. USA. 98:974-979, 2001).

For other TGF-β superfamily members, accessory or co-receptors also playan important regulatory role to promote or inhibit ligand binding (Shiand Massague, Cell 113:685-700, 2003; Lopez-Casillas et al., Cell73:1435-1444, 1993; Shen and Schier, Trends Genet. 16:303-309, 2000;Cheng et al., Genes Dev. 17:31-36, 2003; Gray et al., Proc. Natl. Acad.Sci. USA 100:5193-5198, 2003). Recently, we identified DRAGON(RGMb) asthe first known co-receptor for BMP signaling (Samad et al., J. Biol.Chem. 280:14122-14129, 2005). We therefore investigated whether anotherRGM family member, RGMa, was similarly involved in the BMP signalingpathway. Here, we have demonstrated that RGMa is a BMP co-receptor whichenhances cellular responses to BMP, but not TGF-β.

Although transfection of RGMa into LLC-PK1 cells enhanced BMP signaltransduction without exogenously added ligand, our results indicate thatthis process is ligand-dependent, which is supported by the fact thatRGMa-mediated BMP signaling was inhibited by noggin, a soluble BMPinhibitor which binds and sequesters BMP ligands, preventing access toBMP receptors (Balemans and Van Hul Dev. Biol. 250:231-250, 2002; Groppeet al., Nature 420:636-642, 2002). However, this does not pinpoint theendogenous ligand(s) responsible for RGMa-mediated BMP signaling inthese cells, since noggin has been shown to bind and antagonize severalBMPs, including BMP-2, BMP-4, and BMP-7, as well as some other TGF-βsuperfamily members, including growth and differentiation factor 5(Balemans and Van Hul Dev. Biol. 250:231-250, 2002; Groppe et al.,Nature 420:636-642, 2002; Zimmerman et al., Cell 86:599-606, 1996;Merino et al., Dev. Biol. 206:33-45, 1999). Our findings that aneutralizing antibody to BMP-2 and BMP-4 also inhibited RGMa-mediatedBMP signaling suggest that the major endogenous ligand(s) in these cellsmay be BMP-2 and/or BMP-4. Indeed, RT-PCR confirmed that these cells doendogenously express both BMP-2 and BMP-4. However, this does notpreclude the possibility that some portion of the signaling is relatedto other BMP ligands. Indeed, the manufacturer of this neutralizingantibody does report some minimal cross-reactivity to other BMP ligands.Additionally, this antibody did not completely inhibit RGMa-mediated BMPsignaling to baseline levels. Further evidence for the role of RGMa as aco-receptor for BMP-2 and/or BMP-4 is provided by binding andcrosslinking studies of purified RGMa.Fc in solution. These assays allowthe determination of the binding properties of single types of receptorsand combinations of receptors in isolation, avoiding the presence of anyconfounding co-expressed accessory proteins that may also be present atthe cell surface (del Re et al., J. Biol. Chem. 279:22765-22772, 2004).Here, RGMa.Fc bound directly and specifically to ¹²⁵I-BMP-2 and¹²⁵I-BMP-4, and binding was competitively inhibited by excess cold BMP-2and BMP-4, but not BMP-7 or TGFb1. RGMa.Fc also formed a complex withthe BMP type I receptor ALK6.Fc, and the presence of RGMa.Fc incombination with ALK6.Fc increased binding to ¹²⁵I-BMP-2 compared withALK6.Fc alone. No further increase in binding was seen with the additionof the BMP type II receptor.Fc (data not shown). Although this increasedbinding was additive, not synergistic, the fact that RGMa.Fc formed acomplex with ALK6.Fc indicates that RGMa associates with BMP type Ireceptors that on the cell surface, thereby increasing overall bindingof BMP ligands to the receptor complex and enhancing BMP signaltransduction. While the binding and crosslinking experiments wereperformed using RGMa.Fc and BMP receptor.Fc fusion proteins in solution,support for the role of GPI-anchored, cell-surface RGMa in the BMPsignaling pathway is provided by our findings that RGMa-mediated BMPsignaling was inhibited by dominant negative BMP type I receptors and bydominant negative Smad1, using a BMP-responsive luciferase reporterassay in cell culture. Additionally, transfection of RGMa into LLC-PK1cells increased phosphorylation of endogenous Smad1/5/8, and increasedexpression of endogenous Id1 protein, an important target gene of BMPsignaling in many tissues (Hollnagel et al., J. Biol. Chem.274:19838-19845, 1999; Korchynskyi and ten Dijke J. Biol. Chem.277:4883-4891, 2002; Lopez-Rovira et al., J. Biol. Chem. 277:3176-3185,2002; Miyazono and Miyazawa, Sci STKE. 2002:PE40, 2002; ten Dijke etal., Mol. Cell. Endocrinol. 211:105-113, 2003). The physiologic role ofendogenous RGMa as a BMP co-receptor in vivo is indicated by RGMaexpression in spinal cord neurons along with nuclear accumulation ofp-Smad1/5/8, indicative of BMP signal transduction in these cells.Interestingly, RGMa also acts as a cell survival factor by binding tothe receptor neogenin and inhibiting neogenin's pro-apoptotic activity(Rajagopalan et al., Nat. Cell Biol. 6:756-762, 2004; Matsunaga et al.,Nat. Cell Biol. 6:749-755, 2004). RGMa and DRAGON are members of the RGMfamily of proteins, which also includes the juvenile hemochromatosisgene HJV. RGM family members are highly conserved across vertebrates andinvertebrates and share significant sequence homology as well as similarstructural features (Monnier et al., Nature 419:392-395, 2002;Papanikolaou et al., Nat. Genet. 36:77-82, 2004; Samad et al., J.Neurosci. 24:2027-2036, 2004; Niederkofler et al., J. Neurosci.24:808-818, 2004; Schmidtmer and Engelkamp, Gene Expr. Patterns4:105-110, 2004; Oldekamp et al., Gene Expr Patterns 4:283-288, 2004).HJV also mediates BMP signaling (J. L. Babitt, unpublished data),indicating that these family members all share the ability to act asco-receptors to enhance BMP signals. Our results do not reveal anydifferences between RGMa and DRAGON in regard to their function as BMPco-receptors. Both bind to BMP-2 and BMP-4, but not BMP-7 or othermembers of the TGF-β superfamily. Both signal via the BMP type Ireceptors ALK3 and ALK6 and Smad1. A secreted protein,Kielin/chordin-like protein, has recently been described as a paracrineenhancer of BMP-7 signaling (Lin et al., Nat. Med. 11:387-393, 2005).Thus, multiple enhancing regulatory mechanisms may exist for BMPsignaling to complement the inhibitory regulatory mechanisms which havebeen described (Shi and Massague, Cell 113:685-700, 2003; Balemans andVan Hul Dev. Biol. 250:231-250, 2002; von Bubnoff and Cho Dev. Biol.239:1-14, 2001).

The role of RGM family members may be to differentially increase thesensitivity of cells in which they are expressed to low levels of BMPligands, thus contributing to the tight spatiotemporal regulation of BMPsignal transduction. Northern blot analysis of adult rat tissuesrevealed that endogenous RGMa is expressed in a variety of organs,including heart, brain, lung, liver, skin, kidney, and testis. Whilesome studies have reported a more limited distribution, they werelargely focused on expression in the central nervous system and duringdevelopment (Samad et al., J. Neurosci. 24:2027-2036, 2004; Niederkofleret al., J. Neurosci. 24:808-818, 2004; Schmidtmer and Engelkamp, GeneExpr. Patterns 4:105-110, 2004; Oldekamp et al., Gene Expr Patterns4:283-288; 2004). Additionally, the one previously published Northernblot of RGMa expression in a variety of tissues was limited byunder-exposure (Niederkofler et al., J. Neurosci. 24:808-818, 2004),also a possible explanation for their finding only the larger of the twobands seen in our study. Recent work has also demonstrated a broadertissue distribution for DRAGON, including many tissues throughout thereproductive axis (Xia et al., Endocrinology [Epub ahead of print],2005). We have also found DRAGON expression in the adult rat heart,liver, and kidney by Northern blot (data not shown). While HJVexpression has been described predominantly in the liver, cardiacmuscle, and skeletal muscle (Papanikolaou et al., Nat. Genet. 36:77-82,2004; Samad et al., J. Neurosci. 24:2027-2036, 2004; Niederkofler etal., J. Neurosci. 24:808-818, 2004; Schmidtmer and Engelkamp, Gene Expr.Patterns 4:105-110, 2004; Oldekamp et al., Gene Expr Patterns 4:283-288,2004), one recent study suggested that HJV is also expressed in theadult mouse brain, lung, spleen, kidney, testis, blood, stomach, andintestine (Rodriguez Martinez et al., Haematologica 89:1441-1445, 2004).Thus, RGM family member expression overlaps in a variety of tissues.However, it is possible that the distribution within those tissues isdifferent. For example, in the central nervous system, where RGMa andDRAGON expression have been best characterized, they are predominantlyexpressed in a non-overlapping areas (Samad et al., J. Neurosci.24:2027-2036, 2004; Niederkofler et al., J. Neurosci. 24:808-818, 2004;Schmidtmer and Engelkamp, Gene Expr. Patterns 4:105-110, 2004; Oldekampet al., Gene Expr Patterns 4:283-288, 2004).

Our results define the first family of proteins which function as BMPco-receptors. RGM family members increase the sensitivity of cells inwhich they are expressed to BMP stimulation, and allow these cells torespond earlier or more robustly to a low level of BMP ligand. RGMfamily members thus represent an important addition to the complex arrayof regulatory molecules which help to generate specificity and tightlycoordinate cellular responses to BMP ligands.

Experiments relating to RGMa were carried out as follows.

cDNA Subcloning

cDNA encoding murine RGMa was subcloned into the expression vectorpcDNA4/HisB (Promega, Madison, Wis.). cDNA encoding the extracellulardomain of murine RGMa was amplified by polymerase chain reaction (PCR)and subcloned into the mammalian expression vector pIgplus (R & DSystems, Minneapolis, Minn.) into the restriction sites BamHI andHindIII in-frame with the Fc portion of human immunoglobulin (IgG) togenerate soluble RGMa.Fc fusion protein.

Cell Culture and Transfection

HEK 293 cells and LLC-PK1 cells were obtained from the American TypeCulture Collection (ATCC #CRL1573 and CL-101 respectively) and culturedin Dulbecco's modification of Eagle's medium (DMEM; Cellgro Mediatech,Herndon, Va.) containing 10% fetal bovine serum (FBS; AtlantaBiologicals, Lawrenceville, Ga.). All plasmid transfections wereperformed with Lipofectamine 2000 (Invitrogen, Carlsbad, Calif.)according to manufacturer instructions. Stably transfected cells wereselected and cultured in 2 mg/ml G418 (Cellgro Mediatech).

Luciferase Assay

LLC-PK1 cells were transiently transfected with a TGF-β responsivefirefly luciferase reporter, (CAGA)12MLP-Luc (CAGA-Luc, Dennler et al.,EMBO J. 17:3091-3100, 1998), or a BMP responsive firefly luciferasereporter (BRE-Luc, Korchynskyi, and ten Dijke, J. Biol. Chem277:4883-4891, 2002) (both kindly provided by Peter ten Dijke,Netherlands Cancer Institute), in combination with pRL-TK Renillaluciferase vector (Promega) in a ratio of 10:1 to control fortransfection efficiency, with or without co-transfection with RGMa cDNA.Forty-eight hours after transfection, cells were serum starved in DMEMsupplemented with 1% FBS for 6 hours and treated with varying amounts ofTGF-β, BMP-2, BMP-4, or BMP-7 ligands (R&D Systems) for 16 hours, in theabsence or presence of noggin (R&D Systems) or anti-BMP-2/4 antibody(R&D Systems). Cells were lysed, and luciferase activity was determinedwith the Dual Reporter Assay (Promega) according to the manufacturer'sinstructions. Experiments were performed in duplicate or triplicatewells. Relative luciferase units (R.L.U.) were calculated as the ratioof firefly (reporter) and Renilla (transfection control) luciferasevalues.

Reverse Transcription Polymerase Chain Reaction (RT-PCR)

LLC-PK1 cells were grown to confluence on 6-cm tissue culture plates.Total RNA was isolated using the RNeasy Mini Kit (QIAGEN Inc., Valencia,Calif.) including DNase digestion with the RNase-Free DNase Set (QIAGEN)according to the manufacturer's instructions. First strand cDNAsynthesis was performed using iScript cDNA Synthesis Kit (Bio-Rad,Hercules, Calif.) according to the manufacturer's instructions.Transcripts of BMP-2 were amplified using the forward primer5′-CGTGACCAGACTTTTGGACAC-3′ (SEQ ID NO:16) and reverse primer5′-GGCATGATTAGTGGAGTTCAG-3′ (SEQ ID NO:17). Transcripts of BMP-4 wereamplified using the forward primer 5′-AGCAGCCAAACTATGGGCTA-3′ (SEQ IDNO:18) and reverse primer 5′-TGGTTGAGTTGAGGTGGTCA-3′ (SEQ ID NO:19).

Purification and Characterization of RGMa.Fc

HEK 293 cells stably expressing RGMa.Fc were cultured in DMEMsupplemented with 5% FBS using 175-cm² multifloor flasks (DenvilleScientific, Southplainfield, N.J.). RGMa.Fc was purified from the mediaof stably transfected cells via one-step Protein A affinitychromatography using HiTrap rProtein A FF columns (Amersham Biosciences,Piscataway, N.J.) as described in del Re et al., (J. Biol. Chem.279:22765-22772, 2004). Purified protein was eluted with 100 mMglycine-HCl, pH 3.2 and neutralized with 0.3 M Tris-HCl pH 9 asdescribed in del Re et al, supra).

Purified human RGMa.Fc was subjected to reducing sodiumdodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) usingpre-cast NuPAGE Novex 4-12% Bis-Tris gels (Invitrogen), and gels werestained with Bio-safe Coomassie blue (Bio-Rad) to determine the purityof RGMa.Fc and quantify protein concentration. For Western blotanalysis, gels were electroblotted to polyvinylidene difluoride filter(PDVF) membranes (Bio-Rad). Membranes were blocked with TBS-T (Trisbuffered saline, 0.2% Tween 20) containing 6% milk powder for 1 hour andwashed three times with TBS-T for 10 minutes. Membranes were then probedwith an affinity purified rabbit polyclonal anti-mouse RGMa antibody(α-RGMa) raised against the peptide RMDEEVVNAVEDRDSQGLYLC (SEQ ID NO:20;amino acids 296-316 in the C terminus of mouse RGMa upstream of itshydrophobic tail; GenBank Accession Number NM 177740) (1:2,000) at 4° C.overnight, or with anti-Fc antibody (Jackson ImmunoResearch, West Grove,Pa.) (1:2000) at room temperature for 1 hr in blocking solution.Membranes were washed with TBS-T and incubated with donkey anti-rabbitor anti-goat horseradish peroxidase (HRP)-linked secondary antibody(1:10,000) (Santa Cruz Biotechnology, Santa Cruz, Calif.). Antibodybinding was detected with chemiluminescence reagent (PerkinElmer LifeSciences, Boston, Mass.) and exposed to BioMax XAR film (Kodak,Rochester, N.Y.).

Ligand Iodination

Two mg of carrier free human BMP-2 and BMP-4 ligand (R & D Systems) perreaction was iodinated with [¹²⁵I] by the modified chloramine-T methodas described in Frolik et al., J. Biol. Chem. 259:10995-11000, 1984).

Binding Assay

25 ng purified RGMa.Fc in 1×Tris buffered saline/Casein blocking buffer(BioFX, Owings Mills, Md.) or buffer alone was incubated with ¹²⁵I-BMP-2or ¹²⁵I-BMP-4 in a total volume of 200 ml overnight at 4° C., eitheralone or in the presence of 80 ng cold BMP-2, BMP-4, BMP-7 or TGF-β1 forcompetition assays. For mixing studies, buffer alone, 10 ng purifiedRGMa.Fc alone, 10 ng ALK6.Fc alone (R & D Systems), or 10 ng each ofRGMa.Fc and ALK6.Fc together were incubated in 1×Tris bufferedsaline/Casein blocking buffer with ¹²⁵I-BMP-2 in a total volume of 200ml. The reaction mix was then incubated for 1.5 hrs at 4° C. on proteinA coated plates (Pierce, Rockford, Ill.), plates were washed with washsolution (KPL, Gaithersberg, Md.), and individual wells were countedwith a standard g counter.

DSS Crosslinking in Solution

100 ml ¹²⁵I-BMP-2 or ¹²⁵I-BMP-4 (400,000 C.P.M) was incubated overnightat 4° C. with an equal volume of 20 mM HEPES (pH 7.8), 0.1% bovine serumalbumin, and protease inhibitors (Roche Diagnostics, Mannheim, Germany)alone, or containing 25 ng RGMa.Fc or ALK5.Fc (R & D Systems), in theabsence or presence of 80 ng cold BMP-2 or BMP-4. This mixture wasincubated in the absence or presence of 2.5 mM disuccinimidyl suberate(DSS, Sigma, St. Louis, Mo.) in dimethyl sulfoxide for 2 hr on ice,followed by quenching of DSS activity with 40 mM Tris (pH 7.5) for 15minutes. The mixture was then centrifuged and the supernatant incubatedwith Protein A Sepharose beads (Amersham) at 4° C. for 2 hr toprecipitate hot BMP-2 or -4 bound to RGMa.Fc. Beads were washed withphosphate buffered saline (PBS) and protein eluted by non-reducingLaemmli sample buffer (Bio-Rad). Eluted protein was separated bySDS-PAGE and analyzed by autoradiography.

For receptor crosslinking studies, 200 ng RGMa.Fc, 200 ng ALK6.Fc;and/or 100 ng BMP2 were incubated in 100 ml 20 mM HEPES (pH 7.8), 0.1%bovine serum albumin, and protease inhibitors at 4° C. overnight. Themixtures were then crosslinked with DSS, incubated with protein A beads,and eluted with non-reducing Laemmli sample buffer as described above.The protein complex was then separated by non-reducing SDS-PAGE,electroblotted to PVDF membranes, and analyzed by Western blot usingRGMa antibody (1:2000) as described above for RGMa.Fc.

Measurement of Smad1/5/8 Phosphorylation and Id1 Expression

LLC-PK1 cells plated to 70% confluence were transiently transfected with5 mg RGMa cDNA or empty vector. Twenty-four hours after transfectioncells were incubated in DMEM supplemented with 1% FBS in the absence orpresence of 50 ng/ml BMP2 for 2 hours at 37° C. Cells were sonicated andlysed in 200 mM Tris-Hcl, pH 8.0, 100 mM NaCl, 1 mM EDTA, 0.5% NP-40,and 10% glycerol containing a mixture of protease inhibitors (RocheDiagnostics) for twenty minutes on ice. After centrifugation for 20minutes at 4° C., the supernatant was assayed for protein concentrationby colorimetric assay (BCA kit, Pierce). 30 mg protein was separated bySDS-PAGE and transferred to PVDF membranes. Membranes were probed withα-RGMa (1:2000) as described above for RGMa.Fc. Membranes were strippedin 0.2 M glycine, pH 2.5, 0.5% Tween 20 for 1 hour, and re-probed insuccession with rabbit polyclonal anti-p-Smad1/5/8 antibody(α-p-Smad1/5/8, Cell Signaling, Beverly, Mass.) (1:1000) at 4° C.overnight according to the manufacturer's instructions, rabbitpolyclonal anti-Smad1 antibody (α-Smad1, Upstate Biotechnology, LakePlacid, N.Y.) (1:250) at 4° C. overnight, mouse monoclonal anti-β-actinantibody (α-β-actin, clone AC 15, Sigma) (1:5000) at room temperaturefor 1 hour, and rabbit polyclonal anti-Id1 antibody (α-Id1, C 20, SantaCruz Biotechnology) (1:200) at 4° C. overnight followed by theappropriate HRP-conjugated secondary antibody and chemiluminescencedetection after each as described above. Chemiluminescence wasquantitated using IPLab Spectrum software (Scanalytics, Vienna, Va.).

Northern Blot

Adult rat total RNA was separated on a 1.5% formaldehyde agarose gel andblotted onto GeneScreen Plus membrane (NEN, Boston, Mass.) as describedin Costigan et al. (J. Neurosci. 18:5891-5900, 1998).

Immunohistochemistry

Freshly dissected adult rat lumbar spinal cord was embedded in OCT(Sakura, Tokyo, Japan), frozen on dry ice, cut by cryostat in 16 mmsections, and stored at −80° C. Spinal sections were fixed in 4%paraformaldehyde, washed 3 times in PBS and incubated for 1 hr at roomtemperature in blocking buffer (1% bovine serum albumin, 0.5% Triton Xin PBS). Fixed sections were incubated overnight at 4° C. in blockingbuffer with rabbit polyclonal α-RGMa (1/500) or rabbit polyclonalα-p-Smad1/5/8 (1/100), in combination with mouse monoclonalanti-neuron-specific nuclear protein antibody (α-NeuN, 1/1000)(Chemicon, Temecula, Calif.) to visualize neuronal cell bodies. Sectionswere then washed 3 times in PBS and incubated with cyanin 3(Cy3)-conjugated anti-rabbit and fluorescein isothiocyanate(FITC)-conjugated anti-mouse secondary antibodies (1/200 each, JacksonImmunoResearch) for 1 hr at room temperature. Finally, sections werewashed 3 times in PBS and visualized by fluorescence microscopy.

Enhancing DRAGON Activity for the Treatment or Prevention of aDRAGON-Related Condition

Both the BMP/GDF and the TGF-β/Activin/Nodal branches of the TGF-βsignaling pathway regulate cell proliferation. Normally, TGF-β caninduce an antiproliferative gene response, arresting the cell cycleduring G1. Dysfunction of these pathways has been documented in avariety of cancers. Dysregulation of the TGF-β pathway can occur atalmost any level. The binding of the TGF-β ligands to the various TGF-βreceptors is influenced by soluble protein inhibitors including, forexample, noggin, chordin, caronte, and cerberus. Likewise, thedownstream effects of TGF-β ligand binding are transduced by a proteincascade that includes the R-Smads, Co-Smads, and other co-factors. Theantiproliferative gene responses may involve altering the expression ofa variety of cell cycle regulators (e.g., the cyclin-dependent kinases)or proto-oncogenes (e.g., c-Myc and the retinoblastoma gene). Thus, anintervention that enhances the activity associated with a TGF-βreceptor-ligand binding is a potential point for antiproliferativetherapy.

Based on DRAGON's role as an enhancer of BMP-dependent intracellularsignaling, compounds or therapies which enhance or mimic the biologicalactivity of DRAGON may be therapeutically useful as an antiproliferativeagent. Such candidate compounds include the wild-type DRAGON protein andany compound identified by one of the screening methods describedherein.

DRAGON-enhancing therapy is effective for treating or preventing cancerssuch as pancreatic cancer which have mutations that disable a componentof the TGF-β signaling pathway (Goggins et al., Cancer Res. 58:5329-5332, 1998; Grady et al., Cancer Res. 59: 320-324, 1999; Villanuevaet al., Oncogene 17: 1969-1978, 1998). Cancer-associated mutations havebeen identified in the TGF-β receptors, Smad4, and Smad2. Increasedhepatocyte proliferation, reduced lung and liver apoptosis (Tang et al.Nat. Med. 4: 802-807, 1998), and increased mammary epithelialproliferation and ductal outgrowth in response to hormones(Barcellos-Hoff et al., Breast Cancer Res. 2: 92-99, 2000) has beenobserved in TGF-β1 heterozygous null mice. These studies suggest that anormal and functioning TGF-β signaling pathway has antiproliferativeaction in these cell types. Thus, DRAGON therapy is useful for treatingcancers of these tissues.

Prostate cancer may also be successfully treated by increasing DRAGONbiological activity. Recently, Masuda et al. (Prostate, 59: 101-106,2004) reported that BMP7 expression is highest in the normal prostateglandular tissue and that levels trended lower during the developmentand progression of prostate cancer. These findings implicatedysfunctional TGF-β signaling along the BMP/GDF pathway. Although DRAGONdoes not directly interact with BMP7, both BMP7 and BMP4 (also expressedin normal prostate) signal through common combinations of BMP type I andtype II receptors and intracellular R-Smads. Thus, enhancing BMP4signaling may functionally reverse the deficit in BMP-7 during prostatecancer progression. BMP4 signaling is enhanced by increasing thebiological activity of DRAGON either through administration of a DRAGONprotein, a compound that mimics DRAGON's biological activity, or throughgene therapy techniques. Thus, DRAGON therapy represents an importantavenue for treatment of prostate cancer.

Pulmonary hypertension is another disorder that may be treated byenhancing DRAGON activity. Familial primary pulmonary hypertension is anautosomal dominant disorder caused by a mutation in the BMPRII. Thisdisease, however, has a low (10-20%) penetrance into the affectedpopulation, suggesting that other endogenous mechanism may compensatefor the genetic defect. The histopathologic changes observed in thisautosomal disorder include smooth muscle cell proliferation and in situthrombosis. Similar changes are observed in epigenetic forms ofpulmonary hypertension.

The commonality among all of the diseases described above is a defect inthe BMP/GDF branch of the TGF-β signaling pathway. In view of thepromiscuity of the various BMP type I and type II receptors and theconvergence of several signaling pathways on common intracellulareffectors (e.g., the R-Smads), the common mechanism of increasing DRAGONactivity and enhancing signaling through the remaining and functionalBMP receptors and pathways can effectively treat the genetic andepigenetic forms of each disease.

Recent studies have demonstrated that the addition of BMPs to damagedjoints promotes healing (Edwards, et al., Semin. Arthritis Rheum. 31:33-42, 2001). We have discovered high levels of DRAGON expression in theknee joint of mice (FIG. 16). In view of DRAGON's role as a BMPco-receptor, these findings together indicate that enhanced DRAGONactivity is also be useful for treating disorders of cartilage and bone.The BMPs and the BMP-related factor, GDF5/CDMP1, regulate earlycartilage condensation and developmental joint formation (Storm et al.,Development 122: 3969-3979, 1996; Dev. Biol. 209: 11-27, 1999).Enhancing DRAGON biological activity therefore is useful for promotingchondrogenesis and aiding in healing and repair. This is supported bymolecular epidemiological studies which demonstrate that heterozygousmissense mutations in noggin, an upstream inhibitor of BMP and GDF5,results in two autosomal dominant disorders; proximal symphalangism andmultiple synostoses syndrome (Gong et al. Nat. Genet. 21: 302-304,1999). Furthermore, noggin^(−/−) mice die at or prior to birth withextreme mesenchymal malformations including excessive cartilage and bonyfusions of the appendicular skeleton (Brunet et al. Science 280:1455-1457, 1998; McMahon et al. Genes Dev. 12: 1438-1452, 1998).Mutations in CDMP1 are also associated with human hereditary diseasethat may be treatable with DRAGON therapy. Loss-of-function mutations inCDMP1 have been linked to chondrodysplasias (Hunter-Thompson typeacromesomelic chondrodysplasia), autosomal dominant brachydactyl)-typeC, and Grebe type chondrodysplasia. Thus, a variety of genetic andepigenetic disorders of the bone, cartilage, and joint are amenable totreatment by increasing DRAGON activity.

BMP-7 has been shown to reduce renal injury and fibrosis and act as arenotrophic factor. These effects of BMP-7 have been documented inmodels of acute and chronic renal failure and under conditions forprevention as well as therapy. The renotrophic effects of BMP-7 indicatethat renal disorders and diseases may be treated by increasing DRAGONactivity. Although DRAGON does not appear to directly interact withBMP-7, DRAGON expression is increased following an ischemic kidneyinjury (FIG. 17) it is known that signaling through BMP-2 and BMP-4,known DRAGON targets, converge on common intracellular R-Smads. Thus, itis possible to replicate the therapeutic effects of BMP-7 by increasingDRAGON-enhanced signaling through other BMP members. Renal diseasesamenable to such treatment include ischemic kidney disease and renalfibrosis.

DRAGON is also highly expressed in normal mouse testis (FIG. 18) andova. Thus, male and female infertility that is associated with reducedlevels of DRAGON expression may be treated by increasing DRAGON activityusing any of the methods described here.

Inhibiting DRAGON Activity for the Treatment or Prevention of aDRAGON-Related Condition

Certain pathological conditions are characterized by over-activation ofthe BMP/GDF branch of the TGF-β signaling pathway and, as such, areamenable to treatment by reducing DRAGON biological activity.

We have discovered that DRAGON is present in abnormally high levels inbreast cancer (FIG. 19) and colon cancer (FIG. 20). This findingindicates that breast and colon cancer may be treated or prevented byinhibiting the DRAGON activity. As discussed below, another benefit ofinhibiting DRAGON activity in these forms of cancer is the reduction orprevention in tumor metastasis. Many metastases lodge in the bone, atissue high in endogenous BMP activity. Thus, preventing metastases isan effective method for controlling the spread of secondary tumors whichare often more invasive than the primary ones.

BMP2 is overexpressed in non-small cell lung carcinoma but has little orno expression in normal lung tissue (Langenfeld et al., Carcinogenesis24: 1445-1454, 2003). It appears that BMP2 in lung tissue may act as amorphogen and also stimulate proliferation, differentiation, andmigration, similar to its effects during embryogenesis. Thus, in view ofDRAGON's enhancing effects on BMP2 signaling, non-small cell lungcarcinoma may be treated, or at least prevented from metastasizing, byadministration of a compound that inhibits DRAGON activity. Suchcompounds include, for example, a soluble DRAGON protein fragment (i.e.,a DRAGON protein having a deletion of the GPI anchoring domain), or acandidate compound identified by any of the screening methods describedherein. Therapy may be administered systemically (i.e., by intravenous,intramuscular, or subcutaneous injection) or by inhalation.Alternatively, DRAGON biological activity may be inhibited usingantisense gene therapy or RNAi technology according to previouslypublished methods.

DRAGON Analysis for Cancer Diagnosis

DRAGON expression is altered in may forms of cancer. Accordingly,alterations in DRAGON can be used as a cancer diagnostic or to identifypatients with an increased likelihood of developing cancer. For example,the DRAGON gene of a patient not diagnosed as having cancer may beassessed to determine whether the gene contains an activating or aninactivating mutation. Mutations that result in inappropriately elevatedDRAGON activity indicate an increased likelihood for developing, forexample, a cancer of the breast, colon, testicles, or ovaries. Mutationsthat result in reduced DRAGON activity (e.g., inactivating mutations)indicate an increased likelihood for developing, for example, non-smallcell lung cancer. Assessing the DRAGON gene of a patient can be done byany suitable means known in the art including, for example, sequencingthe gene, or assessing restriction fragment length polymorphisms, singlenucleotide polymorphisms, RT-PCR, and in situ hybridization.

DRAGON expression can also be used to diagnose cancer. For example, thepresence of an altered DRAGON gene (i.e., one with an inactivatingmutation or one that causes constitutive activity) may be assessed in abiological sample used for diagnosis. Such samples include, for example,tissue biopsies. Alternatively, for cancers characterized by alteredDRAGON protein expression, the level of soluble DRAGON expression may bemeasured in a biological fluid such as blood, seminal fluid, or saliva,and compared to the DRAGON levels in healthy individuals. DRAGON may bemeasured at either the RNA or protein level. DRAGON protein levels maybe measured by any appropriate technique known in the art including, forexample, antibody-based assays such as ELISA or Western blotting. DRAGONRNA levels may be measured using any techniques known in the artincluding, for example, RT-PCR or Northern blotting. Biopsy samples mayalso be used to diagnose a cancerous or precancerous condition based onthe DRAGON expression levels. In this case, the diagnosis can be basedon the assessment of the DRAGON protein or RNA levels measured in thebiopsy tissue.

DRAGON Promotes Cellular Adhesion

Cell surface GPI-anchored proteins, including the ephrins and tenascin,act as neuronal and non-neuronal cell adhesion molecules, binding tomolecules expressed on neighboring cells or in the extracellular matrix.To examine whether DRAGON has a cell adhesion role, we measured theamount of adhesion between DRG neurons and HEK293 cells expressingrecombinant DRAGON. DRAGON expression caused nearly a two-fold increasein the number of cultured DRG neurons that adhered to a monolayer ofDRAGON-expressing HEK cells, compared to control HEK cells (FIGS. 21a-21 d). Moreover, pretreatment of DRAGON-expressing HEK cells withPI-PLC resulted in only basal levels of DRG adhesion (FIGS. 21 a-21 d).

We also compared the cellular adhesive effects of DRAGON to otheradhesion substrates. Tissue culture plates were coated with laminin,poly-D-lysine, laminin/polylysine, or DRAGON-Fc. DRG neurons were foundto adhere most to the DRAGON-Fc plates, compared to any other adhesionsubstrate tested. Neuronal adhesion to each of the tested substrates wassignificantly greater than that measured in untreated plates.

To test whether DRAGON promotes adhesion in a homophilic manner, weassessed the interaction between native DRAGON and an AP-tagged DRAGON(DRAGON-AP) that is not detected using the anti-DRAGON antibody.Coexpression of both constructs followed by immunoprecipitationdemonstrated a significant physical interaction between the twoproteins, confirming that DRAGON is capable of homophilic interactions.This finding was confirmed by the addition of DRAGON-GST, alsoundetectable using the anti-DRAGON antibody, to HEK cells expressingrecombinant native DRAGON. Using confocal microscopy, DRAGON-GSTcolocalized with the native DRAGON on the cell surface, further provingthe homophilic DRAGON interaction.

To determine whether the DRAGON homophilic binding requires othermolecules, we have used the previously described bead aggregation assay,commonly used for the analysis of cell adhesion molecules using purifiedproteins (Chappuis-Flament et al., 2001; De Angelis et al., 2001). Thisassay provides an in vitro cell free mimic of cell aggregationexperiments. Green fluorescence and red fluorescence beads coated withDRAGON-Fc were dissociated by sonication before incubation in thepresence of 1 mM calcium at 37° C. and observed microscopically.DRAGON-coated beads aggregated after 2 and 4 hours; whereas, Fc-coatedbeads (negative control) did not. DRAGON, therefore, shares a commonmechanistic feature with the cadherins—both proteins mediate homophilicadhesive interaction in a calcium-dependent manner.

In order to assess the interaction between DRAGON and E-Cadherin,lysates of cultured primary DRG neurons were used forimmunoprecipitation and Western blotting with anti-DRAGON andanti-Cadherin antibodies. These studies demonstrated that DRAGON andE-Cadherin physically interact, suggesting a common cellular function.Additional immunoprecipitation studies indicate that DRAGON facilitatesthe interaction between E-Cadherin and β-Catenin.

It is likely that the DRAGON-dependent adhesive contacts are facilitatedby an interaction of DRAGON with e-cadherin and β-catenin to increasetheir association (FIG. 22). In view of the pro-adhesion properties ofDRAGON, therapies that increase DRAGON activity are useful forinhibiting tumor metastasis. Likewise, patient screening for DRAGONstatus of a malignant tumor can be used as an index of the likelihood ofmetastasis. Additionally, DRAGON-enhancing therapy can also treatlesions of epithelial cells by facilitating wound closure throughepithelial cell adhesion across the lesion site. This therapy is usefulfor treating dermal lesions.

RNA Interference

RNAi is a method for decreasing the cellular expression of specificproteins of interest (reviewed in Tuschl, Chembiochem 2:239-245, 2001;Sharp, Genes & Devel. 15:485-490, 2000; Hutvagner et al., Curr. Opin.Genet. Devel. 12:225-232, 2002; and Hannon, Nature 418:244-251, 2002).In RNAi, gene silencing is typically triggered post-transcriptionally bythe presence of double-stranded RNA (dsRNA) in a cell. This dsRNA isprocessed intracellularly into shorter pieces called small interferingRNAs (siRNAs). The introduction of siRNAs into cells either bytransfection of dsRNAs or through expression of siRNAs using aplasmid-based expression system is increasingly being used to createloss-of-function phenotypes in mammalian cells. Based on the nucleotidesequence of the DRAGON genes; various RNAi molecules may be designed toinhibit DRAGON expression in vivo.

Double-stranded RNA (dsRNA) molecules contain distinct strands of RNAthat have formed a complex, or a single RNA strand that has formed aduplex (small hairpin (sh)RNA). Typically, dsRNAs are about 21 or 22base pairs, but may be shorter or longer if desired. dsRNA can be madeusing standard techniques (e.g., chemical synthesis or in vitrotranscription). Kits are available, for example, from Ambion (Austin,Tex.) and Epicentre (Madison, Wis.). Methods for expressing dsRNA inmammalian cells are described in Brummelkamp et al. Science 296:550-553,2002; Paddison et al: Genes & Devel. 16:948-958, 2002. Paul et al.Nature Biotechnol. 20:505-508, 2002; Sui et al. Proc. Natl. Acad. Sci.USA 99:5515-5520, 2002; Yu et al. Proc. Natl. Acad. Sci. USA99:6047-6052, 2002; Miyagishi et al. Nature Biotechnol. 20:497-500,2002; and Lee et al. Nature Biotechnol. 20:500-505 2002, each of whichis hereby incorporated by reference.

Small hairpin RNAs consist of a stem-loop structure with optional 3′UU-overhangs. While there may be variation, stems can range from 21 to31 base pairs (desirably 25 to 29 bp), and the loops can range from 4 to30 base pairs (desirably 4 to 23 base pairs). For expression of shRNAswithin cells, plasmid vectors containing, for example, either thepolymerase III H1-RNA or U6 promoter, a cloning site for the stem-loopedRNA insert, and a 4-5-thymidine transcription termination signal can beemployed. The Polymerase III promoters generally have well-definedinitiation and stop sites and their transcripts lack poly(A) tails. Thetermination signal for these promoters is defined by the polythymidinetract, and the transcript is typically cleaved after the second uridine.Cleavage at this position generates a 3′ UU overhang in the expressedshRNA, which is similar to the 3′ overhangs of synthetic siRNAs.Additional methods for expressing the shRNA in mammalian cells aredescribed in the references cited above.

We have designed, developed, and used a lentiviral expression vector toexpress shRNA from the human U6 snRNA promoter (Cellogenetics, Inc.) asshown in FIG. 23. These data demonstrate that the DRAGON gene may besilenced using shRNA technology.

siRNA

Short twenty-one to twenty-five nucleotide double stranded RNAs areeffective at down-regulating gene expression in vitro, for example, inmammalian tissue culture cell lines (Elbashir et al., Nature 411:494-498, 2001). Alternatively, siRNAs can be injected into an animal,for example, into the blood stream as described by McCaffrey et al.,(Nature 418: 38-9, 2002).

siRNAs have been shown to effectively downregulate viral gene expression(Coburn et al. J. Virol. 76: 9225-31, 2002; Bitko et al. BMC Microbiol.1:34, 2001; Ge et al., Proc. Natl. Acad. Sci. USA 100: 2718-23, 2003;Gitlin et al., Nature 418: 430-4, 2002).

siRNAs and antisense oligonucleotides have also been used effectively invivo (U.S. Patent Publication 20030153519, McCaffrey et al., Nature 418:38-39, 2002; McCaffrey et al., Hepatology. 38:503-8, 2003).

Methods for producing siRNAs are standard in the art. For example, thesiRNA can be chemically synthesized or recombinantly produced. Forexample, short sense and antisense RNA oligomers can be synthesized andannealed to form double-stranded RNA structures with 2-nucleotideoverhangs at each end (Caplen, et al. Proc. Natl. Acad. Sci. USA,98:9742-9747, 2001; Elbashir, et al. EMBO J., 20:6877-88, 2001). Thesedouble-stranded siRNA structures can then be directly introduced tocells, either by passive uptake or a delivery system of choice, such asdescribed below.

In some embodiments, siRNAs are generated by processing longerdouble-stranded RNAs, for example, in the presence of the enzyme dicerunder conditions in which the dsRNA is processed to RNA molecules ofabout 21 to about 23 nucleotides.

The siRNA molecules can be purified using a number of techniques knownto those of skill in the art. For example, gel electrophoresis can beused to purify siRNAs. Alternatively, non-denaturing methods, such asnon-denaturing column chromatography, can be used to purify the siRNA.In addition, chromatography (e.g., size exclusion chromatography),glycerol gradient centrifugation, affinity purification with antibodycan be used to purify siRNAs.

In preferred embodiments, at least one strand of the siRNA molecules hasa 3′ overhang from about 1 to about 6 nucleotides in length, though itmay be from 2 to 4 nucleotides in length. More preferably, the 3′overhangs are 1-3 nucleotides in length. In other embodiments, onestrand has a 3′ overhang and the other strand is blunt-ended or also hasan overhang. The length of the overhangs may be the same or differentfor each strand. In order to further enhance the stability of the siRNA,the 3′ overhangs can be stabilized against degradation. In oneembodiment, the RNA is stabilized by including purine nucleotides, suchas adenosine or guanosine nucleotides. Alternatively, substitution ofpyrimidine nucleotides by modified analogues, e.g., substitution ofuridine nucleotide 3′ overhangs by 2′-deoxythyinidine is tolerated anddoes not affect the efficiency of RNAi. The absence of a 2′ hydroxylsignificantly enhances the nuclease resistance of the overhang in tissueculture medium and may be beneficial in vivo.

In some embodiments, the RNAi construct is in the form of a hairpinstructure. The hairpin RNAs can be synthesized exogenously or can beformed by transcribing from RNA polymerase III promoters in vivo.Examples of making and using such hairpin RNAs for gene silencing inmammalian cells are described in, for example, Paddison et al., GenesDev, 2002, 16:948-58; McCaffrey et al., Nature, 2002, 418:38-9; McManuset al., RNA, 2002, 8:842-50; Yu et al., Proc Natl. Acad. Sci. USA, 2002,99:6047-52). Preferably, hairpin RNAs are engineered in cells or inanimals to ensure continuous and stable suppression of a desired gene.It is known in the art that siRNAs can be produced by processing ahairpin RNA in the cell.

In other embodiments, a plasmid is used to deliver the double-strandedRNA, e.g., as a transcriptional product. In such embodiments, theplasmid is designed to include a “coding sequence” for each of the senseand antisense strands of the RNAi construct. The coding sequences can bethe same sequence, e.g., flanked by inverted promoters, or can be twoseparate sequences each under transcriptional control of separatepromoters. After the coding sequence is transcribed, the complementaryRNA transcripts base-pair to form the double-stranded RNA. PCTapplication WO01/77350 describes an exemplary vector for bi-directionaltranscription of a transgene to yield both sense and antisense RNAtranscripts of the same transgene in a eukaryotic cell.

Methods for the production and therapeutic administration of siRNAs forin vivo therapies are described in U.S. Patent Publications:20030180756, 2003/0157030, and 20030170891. Methods describing thesuccessful in vivo use of siRNAs are described by Sang et al. (NatureMedicine 9: 347-351, 2003).

Gene Therapy

Gene therapy is another therapeutic approach for modulating DRAGONactivity in a patient. Heterologous nucleic acid molecules, encoding forexample a DRAGON anti-sense nucleic acid, a biologically active DRAGONprotein, a soluble DRAGON protein, or a DRAGON fusion protein, can bedelivered to the target cell of interest. The nucleic acid moleculesmust be delivered to those cells in a form in which they can be taken upby the cells and so that sufficient levels of protein can be produced toprovide a therapeutic benefit.

Transducing viral (e.g., retroviral, adenoviral, and adeno-associatedviral) vectors can be used for somatic cell gene therapy, especiallybecause of their high efficiency of infection and stable integration andexpression (see, e.g., Cayouette et al., Human Gene Therapy 8:423-430,1997; Kido et al., Current Eye Research 15:833-844, 1996; Bloomer etal., J. Virology 71:6641-6649, 1997; Naldini et al., Science272:263-267, 1996; and Miyoshi et al., Proc. Natl. Acad. Sci. USA94:10319, 1997). For example, a full length gene, or a portion thereof,can be cloned into a retroviral vector and expression can be driven fromits endogenous promoter, from the retroviral long terminal repeat, orfrom a promoter specifically expressed in a target cell type of interest(e.g., a neoplastic cell). Other viral vectors that can be used include,for example, a vaccinia virus, a bovine papilloma virus, or a herpesvirus, such as Epstein-Barr Virus (also see, for example, the vectors ofMiller, Human Gene Therapy 15-14, 1990; Friedman, Science 244:1275-1281,1989; Eglitis et al., BioTechniques 6:608-614, 1988; Tolstoshev et al.,Curr. Opin. Biotechnol. 1:55-61, 1990; Sharp, Lancet 337:1277-1278,1991; Cornetta et al., Nuc. Acid Res. Mol. Biol. 36:311-322, 1987;Anderson, Science 226:401-409, 1984; Moen, Blood Cells 17:407-416, 1991;Miller et al., Biotechnology 7:980-990, 1989; Le Gal La Salle et al.,Science 259:988-990, 1993; and Johnson, Chest 107:77 S-83S, 1995).Retroviral vectors are particularly well developed and have been used inclinical settings (Rosenberg et al., N. Engl. J. Med. 323:370, 1990;U.S. Pat. No. 5,399,346).

Non-viral approaches can also be employed for the introduction oftherapeutic nucleic acids to target cells of a patient. For example, anucleic acid molecule can be introduced into a cell by administering thenucleic acid in the presence of lipofection (Felgner et al., Proc. Natl.Acad. Sci. USA 84:7413, 1987; Ono et al., Neurosci. Lett. 17:259, 1990;Brigham et al., Am. J. Med. Sci. 298:278, 1989; Staubinger et al., Meth.Enzymol. 101:512, 1983), asialoorosomucoid-polylysine conjugation (Wu etal., J. Biol. Chem. 263:14621, 1988; Wu et al, J. Biol. Chem. 264:16985,1989), or by micro-injection under surgical conditions (Wolff et al.,Science 247:1465, 1990). Preferably the nucleic acids are administeredin combination with a liposome and protamine.

Gene transfer can also be achieved using non-viral means involvingtransfection in vitro. Such methods include the use of calciumphosphate, DEAE dextran, electroporation, and protoplast fusion.Liposomes can also be potentially beneficial for delivery of DNA into acell. Transplantation of normal genes into the affected tissues of apatient can also be accomplished by transferring a normal nucleic acidinto a cultivatable cell type ex vivo (e.g., an autologous orheterologous primary cell or progeny thereof), after which the cell (orits descendants) are injected into a targeted tissue.

cDNA expression for use in gene therapy methods can be directed from anysuitable promoter (e.g., an endocan promoter, Flt-1 promoter, or othertumor endothelial promoter identified using the methods describedherein), and regulated by any appropriate mammalian regulatory element.For example, if desired, an enhancers known to preferentially directgene expression in a tumor endothelial cell, (e.g., the 300 base pairTie-2 intronic enhancer element described herein) can be used to directthe expression of a nucleic acid. The enhancers used can include,without limitation, those that are characterized as tissue- orcell-specific enhancers. Alternatively, if a genomic clone is used as atherapeutic construct, regulation can be mediated by the cognateregulatory sequences or, if desired, by regulatory sequences derivedfrom a heterologous source, including any of the promoters or regulatoryelements described above.

Another therapeutic approach included in the invention involvesadministration of a recombinant nuclear encoded mitochondrial metabolismor proteasomal polypeptide, either directly to the site of a potentialor actual disease-affected tissue (for example, by injection into theventricles of the brain or into the cerebrospinal fluid) or systemically(for example, by any conventional recombinant protein administrationtechnique). The dosage of the administered protein depends on a numberof factors, including the size and health of the individual patient. Forany particular subject, the specific dosage regimes should be adjustedover time according to the individual need and the professional judgmentof the person administering or supervising the administration of thecompositions. Generally, between 0.1 mg and 100 mg, is administered perday to an adult in any pharmaceutically acceptable formulation.

A desired mode of gene therapy is to provide the polynucleotide in sucha way that it will replicate inside the cell, enhancing and prolongingthe desired effect. Thus, the polynucleotide is operably linked to asuitable promoter, such as the natural promoter of the correspondinggene, a heterologous promoter that is intrinsically active in targetcell, or a heterologous promoter that can be induced by a suitableagent.

Identification of Candidate Compounds for Treatment of DRAGON-RelatedConditions

A candidate compound that is beneficial in the treatment, stabilization,or prevention of a DRAGON-related condition can be identified by themethods of the present invention. A candidate compound can be identifiedfor its ability to affect (increase or decrease) the biological activityof a DRAGON protein or the expression of a DRAGON gene or to modulateits action. Compounds that are identified by the methods of the presentinvention that increase the biological activity or expression levels ofa DRAGON protein or that compensate for the loss of DRAGON proteinactivity or gene expression, for example, due to loss of the DRAGON genedue to a genetic lesion, can be used in the treatment or prevention of aDRAGON-related condition. Compound that are identified by these methodsthat reduce DRAGON biological activity or expression levels may also beused as therapeutics for DRAGON-related conditions characterized byinappropriately high levels of DRAGON biological activity. ElevatedDRAGON biological activity in a disease state may result fromover-expression a DRAGON gene, elevated levels of an activatingpost-translational modification of a DRAGON protein, or throughinteractions of other molecules or cellular components with the DRAGONprotein. A candidate compound identified by the present invention canmimic, activate, or inhibit the biological activity of a DRAGON protein,bind a DRAGON protein, modulate (e.g., increase or decrease)transcription of a DRAGON gene, or modulate translation of a DRAGONmRNA.

One method for evaluating the ability of candidate compounds to modulateDRAGON biological activity is by measuring the binding between DRAGONand a TGF-β signaling pathway member (e.g., a BMP ligand or a type I ortype II BMP receptor). Compounds that modulate this binding interactionare expected to modulate DRAGON biological activity. Such screeningmethods may be performed in cell-based or cell-free assay systems.

Another method for evaluating the ability of candidate compounds tomodulate DRAGON biological activity, in a cell-based assay, is toprovide cells that express DRAGON and a TGF-β signaling pathway member(e.g., a type I or type II BMP receptor, or another intracellularpathway member), and contact the cell with a candidate compound.Compounds that modulate (increase or decrease) the level of TGF-βpathway activation may be useful for treating DRAGON-related conditions.TGF-β pathway activation may be measured in cells expressing aheterologous reporter gene construct, as described below, or may bemeasured by any other appropriate technique including, for example,measuring the phosphorylation levels of intracellular TGF-β signalingpathway members such as the Smads.

Any number of methods are available for carrying out screening assays toidentify new candidate compounds that promote or inhibit the expressionof a DRAGON gene. In one working example, candidate compounds are addedat varying concentrations to the culture medium of cultured cellsexpressing one of the DRAGON nucleic acid sequences of the invention.Gene expression is then measured, for example, by microarray analysis,Northern blot analysis (Ausubel et al., supra), or RT-PCR, using anyappropriate fragment prepared from the nucleic acid molecule as ahybridization probe. The level of DRAGON gene expression in the presenceof the candidate compound is compared to the level measured in a controlculture medium lacking the candidate compound. A compound which promotesa change (increase or decrease) in the expression of a DRAGON gene isconsidered useful in the invention and may be used as a therapeutic totreat a human patient.

In another working example, the effect of candidate compounds may bemeasured at the level of DRAGON protein production using the samegeneral approach and standard immunological techniques, such as Westernblotting or immunoprecipitation with an antibody specific for a DRAGONprotein. For example, immunoassays may be used to detect or monitor theexpression of at least one of the polypeptides of the invention in anorganism. Polyclonal or monoclonal antibodies that are capable ofbinding to a DRAGON protein may be used in any standard immunoassayformat (e.g., ELISA, Western blot, or RIA assay) to measure the level ofthe protein. In some embodiments, a compound that promotes a change(increase or decrease) in DRAGON expression or biological activity isconsidered particularly useful.

Expression of a reporter gene that is operably linked to the promoter ofa TGF-β signaling pathway member, (e.g., a promoter from a DRAGON gene,a BMP ligand gene, or a BMP type I or type II receptor gene) can also beused to identify a candidate compound for treating or preventing aDRAGON-related condition. Assays employing the detection of reportergene products are extremely sensitive and readily amenable toautomation, hence making them ideal for the design of high-throughputscreens. Assays for reporter genes may employ, for example,colorimetric, chemiluminescent, or fluorometric detection of reportergene products. Many varieties of plasmid and viral vectors containingreporter gene cassettes are easily obtained. Such vectors containcassettes encoding reporter genes such as lacZ/β-galactosidase, greenfluorescent protein, and luciferase, among others. A genomic DNAfragment carrying a TGF-β signaling pathway member-specific (e.g.,DRAGON-specific) transcriptional control region (e.g., a promoter and/orenhancer) is first cloned using standard approaches (such as thosedescribed by Ausubel et al. (supra). The DNA carrying the TGF-βsignaling pathway member transcriptional control region is theninserted, by DNA subcloning, into a reporter vector, thereby placing avector-encoded reporter gene under the control of that transcriptionalcontrol region. The activity of the TGF-β signaling pathway membertranscriptional control region operably linked to the reporter gene canthen be directly observed and quantified as a function of reporter geneactivity in a reporter gene assay.

In one embodiment, for example, the DRAGON transcriptional controlregion could be cloned upstream from a luciferase reporter gene within areporter vector. This could be introduced into the test cells, alongwith an internal control reporter vector (e.g., a lacZ gene under thetranscriptional regulation of the (β-actin promoter). After the cellsare exposed to the test compounds, reporter gene activity is measuredand DRAGON reporter gene activity is normalized to internal controlreporter gene activity.

In addition, candidate compounds may be identified using any of theDRAGON fusion proteins described above (e.g., as compounds that bind tothose fusion proteins), or by any of the two-hybrid or three-hybridassays described above.

A candidate compound identified by the methods of the present inventioncan be from natural as well as synthetic sources. Those skilled in thefield of drug discovery and development will understand that the precisesource of test extracts or compounds is not critical to the methods ofthe invention. Examples of such extracts or compounds include, but arenot limited to, plant-, fungal-, prokaryotic-, or animal-based extracts,fermentation broths, and synthetic compounds, as well as modification ofexisting compounds. Numerous methods are also available for generatingrandom or directed synthesis (e.g., semi-synthesis or total synthesis)of any number of chemical compounds, including, but not limited to,saccharide-, lipid-, peptide-, and nucleic acid-based compounds.Synthetic compound libraries are commercially available from BrandonAssociates (Merrimack, N.H.) and Aldrich Chemical (Milwaukee, Wis.).Alternatively, libraries of natural compounds in the form of bacterial,fungal, plant, and animal extracts are commercially available from anumber of sources, including Biotics (Sussex, UK), Xenova (Slough, UK),Harbor Branch Oceangraphics Institute (Ft. Pierce, Fla.), and PharmaMar,U.S.A. (Cambridge, Mass.). In addition, natural and syntheticallyproduced libraries are produced, if desired, according to methods knownin the art, e.g., by standard extraction and fractionation methods.Furthermore, if desired, any library or compound is readily modifiedusing standard chemical, physical, or biochemical methods.

Synthesis of DRAGON Proteins

Nucleic acids that encode a DRAGON protein or fragment thereof may beintroduced into various cell types or cell-free systems for expression,thereby allowing purification of the DRAGON protein for biochemicalcharacterization, large-scale production, antibody production, andpatient therapy.

Eukaryotic and prokaryotic DRAGON expression systems may be generated inwhich a DRAGON gene sequence is introduced into a plasmid or othervector, which is then used to transform living cells. Constructs inwhich the DRAGON cDNA contains the entire open reading frame inserted inthe correct orientation into an expression plasmid may be used forprotein expression. Alternatively, portions of the DRAGON gene sequence,including wild-type or mutant DRAGON sequences, may be inserted.Prokaryotic and eukaryotic expression systems allow various importantfunctional domains of the DRAGON protein to be recovered, if desired, asfusion proteins, and then used for binding, structural, and functionalstudies and also for the generation of appropriate antibodies. Typicalexpression vectors contain promoters that direct the synthesis of largeamounts of mRNA corresponding to the inserted DRAGON nucleic acid in theplasmid-bearing cells. They may also include a eukaryotic or prokaryoticorigin of replication sequence allowing for their autonomous replicationwithin the host organism, sequences that encode genetic traits thatallow vector-containing cells to be selected for in the presence ofotherwise toxic drugs, and sequences that increase the efficiency withwhich the synthesized mRNA is translated. Stable long-term vectors maybe maintained as freely replicating entities by using regulatoryelements of, for example, viruses (e.g., the OriP sequences from theEpstein Barr Virus genome). Cell lines may also be produced that haveintegrated the vector into the genomic DNA, and in this manner the geneproduct is produced on a continuous basis.

Expression of foreign sequences in bacteria, such as Escherichia coli,requires the insertion of the DRAGON nucleic acid sequence into abacterial expression vector. Such plasmid vectors contain severalelements required for the propagation of the plasmid in bacteria, andfor expression of the DNA inserted into the plasmid. Propagation of onlyplasmid-bearing bacteria is achieved by introducing, into the plasmid,selectable marker-encoding sequences that allow plasmid-bearing bacteriato grow in the presence of otherwise toxic drugs. The plasmid alsocontains a transcriptional promoter capable of producing large amountsof mRNA from the cloned gene. Such promoters may be (but are notnecessarily) inducible promoters that initiate transcription uponinduction. The plasmid also preferably contains a polylinker to simplifyinsertion of the gene in the correct orientation within the vector.

Mammalian cells can also be used to express a DRAGON protein. Stable ortransient cell line clones can be made using DRAGON expression vectorsto produce DRAGON proteins in a soluble (truncated and tagged) ormembrane anchored (native) form. Appropriate cell lines include, forexample, COS, HEK293T, CHO, or NIH cell lines.

Once the appropriate expression vectors containing a DRAGON gene,fragment, fusion, or mutant are constructed, they are introduced into anappropriate host cell by transformation techniques, such as, but notlimited to, calcium phosphate transfection, DEAE-dextran transfection,electroporation, microinjection, protoplast fusion, or liposome-mediatedtransfection. The host cells that are transfected with the vectors ofthis invention may include (but are not limited to) E. coli or otherbacteria, yeast, fungi, insect cells (using, for example, baculoviralvectors for expression in SF9 insect cells), or cells derived from mice,humans, or other animals. In vitro expression of a DRAGON protein,fusion, polypeptide fragment, or mutant encoded by cloned DNA may alsobe used. Those skilled in the art of molecular biology will understandthat a wide variety of expression systems and purification systems maybe used to produce recombinant DRAGON proteins and fragments thereof.Some of these systems are described, for example, in Ausubel et al.(supra).

Once a recombinant protein is expressed, it can be isolated from celllysates using protein purification techniques such as affinitychromatography. Once isolated, the recombinant protein can, if desired,be purified further by e.g., by high performance liquid chromatography(HPLC; e.g., see Fisher, Laboratory Techniques In Biochemistry AndMolecular Biology, Work and Burdon, Eds., Elsevier, 1980).

Polypeptides of the invention, particularly short DRAGON fragments canalso be produced by chemical synthesis (e.g., by the methods describedin Solid Phase Peptide Synthesis, 2nd ed., 1984, The Pierce ChemicalCo., Rockford, Ill.).

Chimeric DRAGON Proteins

Also included in the invention are DRAGON proteins fused to heterologoussequences, such as cytotoxic moieties (e.g., saporin) and detectablemarkers (for example, proteins that may be detected directly orindirectly such as green fluorescent protein, hemagglutinin, or alkalinephosphatase), DNA binding domains (for example, GAL4 or LexA), geneactivation domains (for example, GAL4 or VP16), purification tags, orsecretion signal peptides. These fusion proteins may be produced by anystandard method. For production of stable cell lines expressing a DRAGONfusion protein, PCR-amplified DRAGON nucleic acids may be cloned intothe restriction site of a derivative of a mammalian expression vector.For example, KA, which is a derivative of pcDNA3 (Invitrogen, Carlsbad,Calif.) contains a DNA fragment encoding an influenza virushemagglutinin (HA). Alternatively, vector derivatives encoding othertags, such as c-myc or poly Histidine tags, can be used.

The DRAGON expression construct may be co-transfected, with a markerplasmid, into an appropriate mammalian cell line (e.g. COS, HEK293T, orNIH 3T3 cells) using, for example, Lipofectamine™ (Gibco-BRL,Gaithersburg, Md.) according to the manufacturer's instructions, or anyother suitable transfection technique known in the art. Suitabletransfection markers include, for example, β-galactosidase or greenfluorescent protein (GFP) expression plasmids or any plasmid that doesnot contain the same detectable marker as the DRAGON fusion protein. TheDRAGON-expressing cells can be sorted and further cultured, or thetagged DRAGON can be purified.

In one particular example, a DRAGON open reading frame (ORF) wasamplified by polymerase chain reaction (PCR) using standard techniquesand primers containing restriction sites (e.g. Sal I sites). The topstrand primer consisted of the sequence 5′-ATA AGC TTA TGG GCG TGA GAGCAG CAC CTT CC-3′ (SEQ ID NO:21) and the bottom strand primer consistedof the sequence 5′-GAA GTC GAC GAA ACA ACT CCT ACA AAA AC-3′ (SEQ IDNO:22). DRAGON cDNA was also amplified without the signal peptide andsubcloned into a vector (pSecTagHis) having a strong secretion signalpeptide. The same bottom strand primer was used (SEQ ID NO:22); however,the top strand primer was substituted for one having the sequence 5′-CTCAAG CTT CAG CCT ACT CAA TGC CGA ATC-3′ (SEQ ID NO:23).

In another example, DRAGON-Fc was generated by subcloning DRAGON cDNAwithout its GPI anchor into the mammalian expression vector pIgplus (R&DSystems, Minneapolis, Minn.) in frame with the Fc portion of the humanIgG. This allowed expression of a soluble DRAGON-Fc fusion protein.DRAGON-Fc collected in the media of stably transfected HEK293 cells waspurified using HiTrap Protein A Affinity Columns (Amersham Biosciences)and eluted with 100 mM glycine-HCl, pH 3.0. The elution fraction wasneutralized with 0.3 mM Tris-HCl, pH 9. Purified DRAGON-Fc wasidentified following electrophoretic separation on an SDS-PAGE gel andimmunoblotting with anti-DRAGON antibody and an anti-Fc antibody.

In another example, we generated DRAGON-alkaline phosphatase (AP) fusionprotein using the mammalian expression vector, pAPtag-5′ (Flanagan etal., Meth. Enzymol. 327:198-210, 2000). When expressed in mammaliancells (e.g. HEK 293), the DRAGON-AP fusion protein is secreted at highlevels into the culture medium and is easily detected by the AP activityassay. The resulting DRAGON-AP fusion protein can be used to screenexpression libraries to identify, clone, sequence, and characterizemolecules which interact with DRAGON, such as cell surface receptors orendogenous DRAGON ligands. Of course, this method is broadly applicableto any number of suitable tags known in the art.

A fusion protein (chimera) of DRAGON protein or fragment and a cytotoxicmoiety may be used for the treatment of cancer. A particularly usefulcytotoxic moiety is saporin, a protein isolated from Saponariaofficinalis (common soapwort) which function as ribosomal inactivatingproteins. Several such saporins are described, for example, Barthelemyet al. (J. Biol. Chem. 268: 6541-6548, 1993). One particularly usefulsaporin is represented by Genbank Accession No. CAA48885. When using aproteinaceous cytotoxic moiety, such as saporin, the polynucleotidecoding sequence may be directly linked, in frame, to either the 5′ orthe 3′ terminus of the DRAGON coding sequence. Preferably, the cytotoxicmoiety is linked to the 5′ (N-terminus) of the DRAGON sequence in orderto preserve the naturally-occurring GPI anchoring domain at the DRAGONC-terminus. Optionally, a polynucleotide encoding a linker peptide maybe inserted between the two sequences.

Fusion proteins containing a DRAGON protein or fragment and a cytotoxicmoiety (e.g., saporin) may be administered to a patient, for thetreatment of cancer, using any appropriate technique. For example, avector encoding the fusion protein may be administered such that thefusion protein is expressed in the targeted cancer cells or innon-cancerous cells. The DRAGON moiety is used to target the fusionprotein to cancerous cells expressing a high level of a TGF-β signalingpathway member (e.g., a type I or type II BMP receptor) and allow thecytotoxic moiety to affect cell killing. Alternatively, the fusionprotein may be administered systemically or by an intra-tumor injection.

Generation of Anti-DRAGON Antibodies

In order to prepare polyclonal antibodies, DRAGON proteins, fragments,or fusion proteins containing defined portions of a DRAGON protein maybe synthesized in bacterial, fungal, or mammalian cells by expression ofcorresponding DNA sequences in a suitable cloning vehicle. The proteincan be purified, coupled to a carrier protein, mixed with Freund'sadjuvant (to enhance stimulation of the antigenic response in aninnoculated animal), and injected into rabbits or other laboratoryanimals. Following booster injections at bi-weekly intervals, therabbits or other laboratory animals are then bled and the sera isolated.The sera can be used directly or can be purified prior to use by variousmethods, including affinity chromatography employing reagents such asProtein A-Sepharose, antigen-Sepharose, and anti-mouse-Ig-Sepharose. Thesera can then be used to probe protein extracts from DRAGON-expressingtissue electrophoretically fractionated on a polyacrylamide gel toidentify DRAGON proteins. Alternatively, synthetic peptides can be madethat correspond to the antigenic portions of the protein and used toinnoculate the animals. As described above, a polyclonal antibodyagainst mDRAGON was created using, as the immunogenic DRAGON fragment, apolypeptide corresponding to residues 388-405 of SEQ ID NO: 5. Suitableimmunogens for creating anti-hDRAGON antibodies include, for example,the polypeptide sequences encoded by residues 54-72, 277-294, or 385-408of SEQ ID NO: 2.

Alternatively, monoclonal antibodies may be prepared using DRAGONproteins described above and standard hybridoma technology (see, e.g.,Kohler et al., Nature 256:495, 1975; Kohler et al., Eur. J. Immunol.6:511; 1976; Kohler et al., Eur. J. Immunol. 6:292, 1976; Hammerling etal., In Monoclonal Antibodies and T Cell Hybridomas, Elsevier, New York,N.Y., 1981). Once produced, monoclonal antibodies are also tested forspecific DRAGON protein recognition by Western blot orimmunoprecipitation analysis.

Antibodies of the invention may also be produced using DRAGON amino acidsequences that do not reside within highly conserved regions, and thatappear likely to be antigenic, as analyzed by criteria such as thoseprovided by the Peptide Structure Program (Genetics Computer GroupSequence Analysis Package, Program Manual for the GCG Package, Version7, 1991) using the algorithm of Jameson and Wolf (CABIOS 4:181, 1988).

In Situ Hybridization

The in situ hybridization methods used herein have been describedpreviously (Karchewski et al., J. Comp. Neurol. 413:327, 1999).Hybridization was performed on fresh frozen, mounted tissue sectionsfrom mouse embryo and adult rat dorsal root ganglia (DRG) usingterminally-labeled oligonucleotide probes. Probes had approximately 50%G-C content and were complementary and selective for mDRAGON mRNAs.Probes were 3′-end labeled with ³⁵S-dATP using a terminal transferasereaction and purified through a spin column (Qiagen). Hybridization wasdone under very high stringency conditions such that probe annealingrequired at least 90% sequence identity (Dagerlind et al.,Histochemistry 98:39, 1992).

Briefly, slides were brought to room-temperature and covered with ahybridization solution (50% formamide, 1×Denhardt's solution, 1%sarcosyl, 10% dextran sulphate, 0.02M phosphate buffer, 4×SSC, 200 nMDTT, 500 mg/ml salmon sperm DNA) containing 10⁷ cpm/ml of labeled probe.Slides were incubated in a humidified chamber at 43° C. for 14-18 hours,then washed 4×15 min in 1×SSC at 55° C. In the final rinse, slides werebrought to room temperature, washed in dH₂O, dehydrated in ethanol, andair dried.

Autoradiograms were generated by dipping slides in NTB2 nuclear trackemulsion and storing in the dark at 4° C. Prior to conventionaldeveloping and fixation, sections were allowed to expose for 1-3 weeks,depending on the abundance of transcript. Unstained tissue was viewedunder darkfield conditions using a fiber-optic darkfield stage adapter(MVI), while stained tissue was examined under brightfield conditions.Control experiments using sense probes were conducted to confirm thespecificity of hybridization. The antisense oligonucleotide probe is:

mDRAGON—Specific for Nucleotides 831-879 of SEQ ID NO: 5:

(SEQ ID NO: 24) 5′-TCG CAC AAA CAC TGT GGT GCC TAT GTA GCG GGCATG CAT CTC TAC GTA-3′.

Pharmaceutical Compositions for Increasing DRAGON Biological Activity

The present invention includes the administration of DRAGON for thetreatment or prevention of a DRAGON-related condition. Theadministration of biologically active DRAGON, regardless of its methodof manufacture, restores DRAGON biological activity in a patient lackingendogenous activity of a DRAGON protein due to a loss or reduction ofexpression or biological activity, e.g., by mutation or loss of DRAGONgene or cells that normally express DRAGON.

Peptide agents of the invention, such as DRAGON, can be administered toa subject, e.g., a human, directly or in combination with anypharmaceutically acceptable carrier or salt known in the art.Pharmaceutically acceptable salts may include non-toxic acid additionsalts or metal complexes that are commonly used in the pharmaceuticalindustry. Examples of acid addition salts include organic acids such asacetic, lactic, pamoic, maleic, citric, malic, ascorbic, succinic,benzoic, palmitic, suberic, salicylic, tartaric, methanesulfonic,toluenesulfonic, or trifluoroacetic acids or the like; polymeric acidssuch as tannic acid, carboxymethyl cellulose, or the like; and inorganicacids such as hydrochloric acid, hydrobromic acid, sulfuric acidphosphoric acid, or the like. Metal complexes include zinc, iron, andthe like. One exemplary pharmaceutically acceptable carrier isphysiological saline. Other physiologically acceptable carriers andtheir formulations are known to one skilled in the art and described,for example, in Remington's Pharmaceutical Sciences, (19th edition), ed.A. Gennaro, 1995, Mack Publishing Company, Easton, Pa.

Pharmaceutical formulations of a therapeutically effective amount of apeptide agent or candidate compound of the invention, orpharmaceutically acceptable salt-thereof, can be administered orally,parenterally (e.g. intramuscular, intraperitoneal, intravenous, orsubcutaneous injection), or by intrathecal or intracerebroventricularinjection in an admixture with a pharmaceutically acceptable carrieradapted for the route of administration.

Methods well known in the art for making formulations are found, forexample, in Remington's Pharmaceutical Sciences (19th edition), ed. A.Gennaro, 1995, Mack Publishing Company, Easton, Pa. Compositionsintended for oral use may be prepared in solid or liquid forms accordingto any method known to the art for the manufacture of pharmaceuticalcompositions. The compositions may optionally contain sweetening,flavoring, coloring, perfuming, and/or preserving agents in order toprovide a more palatable preparation. Solid dosage forms for oraladministration include capsules, tablets, pills, powders, and granules.In such solid forms, the active compound is admixed with at least oneinert pharmaceutically acceptable carrier or excipient. These mayinclude, for example, inert diluents, such as calcium carbonate, sodiumcarbonate, lactose, sucrose, starch, calcium phosphate, sodiumphosphate, or kaolin. Binding agents, buffering agents, and/orlubricating agents (e.g., magnesium stearate) may also be used. Tabletsand pills can additionally be prepared with enteric coatings.

Liquid dosage forms for oral administration include pharmaceuticallyacceptable emulsions, solutions, suspensions, syrups, and soft gelatincapsules. These forms contain inert diluents commonly used in the art,such as water or an oil medium. Besides such inert diluents,compositions can also include adjuvants, such as wetting agents,emulsifying agents, and suspending agents.

Formulations for parenteral administration include sterile aqueous ornon-aqueous solutions, suspensions, or emulsions. Examples of suitablevehicles include propylene glycol, polyethylene glycol, vegetable oils,gelatin, hydrogenated naphalenes, and injectable organic esters, such asethyl oleate. Such formulations may also contain adjuvants, such aspreserving, wetting, emulsifying, and dispersing agents.

Biocompatible, biodegradable lactide polymer, lactide/glycolidecopolymer, or polyoxyethylene-polyoxypropylene copolymers may be used tocontrol the release of the compounds. Other potentially usefulparenteral delivery systems for the proteins of the invention includeethylene-vinyl acetate copolymer particles, osmotic pumps, implantableinfusion systems, and liposomes.

Liquid formulations can be sterilized by, for example, filtrationthrough a bacteria-retaining filter, by incorporating sterilizing agentsinto the compositions, or by irradiating or heating the compositions.Alternatively, they can also be manufactured in the form of sterile,solid compositions which can be dissolved in sterile water or some othersterile injectable medium immediately before use.

The amount of active ingredient in the compositions of the invention canbe varied. One skilled in the art will appreciate that the exactindividual dosages may be adjusted somewhat depending upon a variety offactors, including the protein being administered, the time ofadministration, the route of administration, the nature of theformulation, the rate of excretion, the nature of the subject'sconditions, and the age, weight, health, and gender of the patient.Generally, dosage levels of between 0.1 μg/kg to 100 mg/kg of bodyweight are administered daily as a single dose or divided into multipledoses. Desirably, the general dosage range is between 250 μg/kg to 5.0mg/kg of body weight per day. Wide variations in the needed dosage areto be expected in view of the differing efficiencies of the variousroutes of administration. For instance, oral administration generallywould be expected to require higher dosage levels than administration byintravenous injection. Variations in these dosage levels can be adjustedusing standard empirical routines for optimization, which are well knownin the art. In general, the precise therapeutically effective dosagewill be determined by the attending physician in consideration of theabove identified factors.

The protein or therapeutic compound of the invention can be administeredin a sustained release composition, such as those described in, forexample, U.S. Pat. No. 5,672,659 and U.S. Pat. No. 5,595,760. The use ofimmediate or sustained release compositions depends on the type ofcondition being treated. If the condition consists of an acute orsubacute disorder, a treatment with an immediate release form will bepreferred over a prolonged release composition. Alternatively, forpreventative or long-term treatments, a sustained released compositionwill generally be preferred.

The protein or therapeutic compound of the present invention can beprepared in any suitable manner. The protein or therapeutic compound canbe isolated from naturally occurring sources, recombinantly produced, orproduced synthetically, or produced by a combination of these methods.The synthesis of short peptides is well known in the art. See e.g.Stewart et al., Solid Phase Peptide Synthesis (Pierce Chemical Co., 2ded., 1984).

Other Embodiments

All publications and patent applications cited in this specification areherein incorporated by reference as if each individual publication orpatent application were specifically and individually indicated to beincorporated by reference. Although the foregoing invention has beendescribed in some detail by way of illustration and example for purposesof clarity of understanding, it will be readily apparent to those ofordinary skill in the art in light of the teachings of this inventionthat certain changes and modifications may be made thereto withoutdeparting from the spirit or scope of the appended claims. Otherembodiments are within the claims.

1. A method for identifying a compound that modulates a TGF-β signalingpathway, said method comprising the steps of: a. providing a samplecomprising a DRAGON protein and a TGF-β signaling pathway member; b.contacting said sample with a candidate compound; and c. assessing thebinding of said DRAGON protein to said TGF-β signaling pathway member insaid sample in the presence of said candidate compound relative tobinding in the absence of said candidate compound, wherein a compoundthat modulates binding of said DRAGON protein to said TGF-β signalingpathway member is identified as a compound that modulates a TGF-βsignaling pathway. 2-41. (canceled)